BMRB Entry 52767

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52767

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Title:
An extended backbone assignment of the monomeric DrBphP CBDPHY construct in the Pr state.
Deposition date:
2024-12-19
Original release date:
2025-08-14
Authors:
Vrhovac, Lidija; Levkovets, Maria; Orekhov, Vladislav; Westenhoff, Sebastian
Citation:

Citation: Vrhovac, Lidija; Levkovets, Maria; Orekhov, Vladislav; Westenhoff, Sebastian. "Refolding of the Deinococcus Radiodurans phytochrome photosensory module and an extended backbone resonance assignment by solution NMR"  PLOS ONE ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 509 residues, 57000 Da.

Natural source:

Natural source:   Common Name: Deinococcus radiodurans   Taxonomy ID: 1299   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Deinococcus radiodurans

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MSRDPLPFFPPLYLGGPEIT TENCEREPIHIPGSIQPHGA LLTADGHSGEVLQMSLNAAT FLGQEPTVLRGQTLAALLPE QWPALQAALPPGCPDALQYR ATLDWPAAGHLSLTVHRVGE LLILEFEPTEAWDSTGPHAL RNAMSALESAPNLRALAEVA TQTVRELTGFDRVMLYKFAP DATGEVIAEARREGLHAFLG HRFPASDIPAQARALYTRHL LRLTADTRAAAVPLDPVLNP QTNAPTPLGGAVLRATSPMH MQYLRNMGVGSSLSVSVVVG GQLWGLIACHHQTPYVLPPD LRTTLEYLGRELSEQVQVKE AADVAAFRQSLREHHARVAL AAAHSLSPHDTLSDPALDLL GLMRAGGLILRFEGRWQTLG EVPPAPAVDALLAWLETQPG ALVQTDALGQLWPAGADLAP SAAGLLAISVGEGWSECLVW LRPELRLEVAWGGATPDQAK DDLGPRHSFDTYLEEKRGYA EPWHPGEIEEAQDLRDTLTG ALEHHHHHH

Data sets:
Data typeCount
13C chemical shifts881
15N chemical shifts277
1H chemical shifts277

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PAS-GAF-PHY1

Entities:

Entity 1, PAS-GAF-PHY 509 residues - 57000 Da.

1   METSERARGASPPROLEUPROPHEPHEPRO
2   PROLEUTYRLEUGLYGLYPROGLUILETHR
3   THRGLUASNCYSGLUARGGLUPROILEHIS
4   ILEPROGLYSERILEGLNPROHISGLYALA
5   LEULEUTHRALAASPGLYHISSERGLYGLU
6   VALLEUGLNMETSERLEUASNALAALATHR
7   PHELEUGLYGLNGLUPROTHRVALLEUARG
8   GLYGLNTHRLEUALAALALEULEUPROGLU
9   GLNTRPPROALALEUGLNALAALALEUPRO
10   PROGLYCYSPROASPALALEUGLNTYRARG
11   ALATHRLEUASPTRPPROALAALAGLYHIS
12   LEUSERLEUTHRVALHISARGVALGLYGLU
13   LEULEUILELEUGLUPHEGLUPROTHRGLU
14   ALATRPASPSERTHRGLYPROHISALALEU
15   ARGASNALAMETSERALALEUGLUSERALA
16   PROASNLEUARGALALEUALAGLUVALALA
17   THRGLNTHRVALARGGLULEUTHRGLYPHE
18   ASPARGVALMETLEUTYRLYSPHEALAPRO
19   ASPALATHRGLYGLUVALILEALAGLUALA
20   ARGARGGLUGLYLEUHISALAPHELEUGLY
21   HISARGPHEPROALASERASPILEPROALA
22   GLNALAARGALALEUTYRTHRARGHISLEU
23   LEUARGLEUTHRALAASPTHRARGALAALA
24   ALAVALPROLEUASPPROVALLEUASNPRO
25   GLNTHRASNALAPROTHRPROLEUGLYGLY
26   ALAVALLEUARGALATHRSERPROMETHIS
27   METGLNTYRLEUARGASNMETGLYVALGLY
28   SERSERLEUSERVALSERVALVALVALGLY
29   GLYGLNLEUTRPGLYLEUILEALACYSHIS
30   HISGLNTHRPROTYRVALLEUPROPROASP
31   LEUARGTHRTHRLEUGLUTYRLEUGLYARG
32   GLULEUSERGLUGLNVALGLNVALLYSGLU
33   ALAALAASPVALALAALAPHEARGGLNSER
34   LEUARGGLUHISHISALAARGVALALALEU
35   ALAALAALAHISSERLEUSERPROHISASP
36   THRLEUSERASPPROALALEUASPLEULEU
37   GLYLEUMETARGALAGLYGLYLEUILELEU
38   ARGPHEGLUGLYARGTRPGLNTHRLEUGLY
39   GLUVALPROPROALAPROALAVALASPALA
40   LEULEUALATRPLEUGLUTHRGLNPROGLY
41   ALALEUVALGLNTHRASPALALEUGLYGLN
42   LEUTRPPROALAGLYALAASPLEUALAPRO
43   SERALAALAGLYLEULEUALAILESERVAL
44   GLYGLUGLYTRPSERGLUCYSLEUVALTRP
45   LEUARGPROGLULEUARGLEUGLUVALALA
46   TRPGLYGLYALATHRPROASPGLNALALYS
47   ASPASPLEUGLYPROARGHISSERPHEASP
48   THRTYRLEUGLUGLULYSARGGLYTYRALA
49   GLUPROTRPHISPROGLYGLUILEGLUGLU
50   ALAGLNASPLEUARGASPTHRLEUTHRGLY
51   ALALEUGLUHISHISHISHISHISHIS

Samples:

sample_1: Phytochrome, [U-13C; U-15N; U-2H], 0.6 mM; sodium chloride 50 mM; sodium phosphate 25 mM; azide 0.02%

sample_conditions_1: ionic strength: 0.075 M; pH: 6.9; pressure: 1 atm; temperature: 318 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
2D 1H-15N TROSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESY TROSYsample_1isotropicsample_conditions_1

Software:

CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks