BMRB Entry 52762

Name: DNAJB1 Y92L
Published: 2025-06-04

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52762

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

DNAJB1 Y92L

Deposition date:

2024-12-17

Original release date:

2025-06-04

Authors:

Hobbs, Billy; Karamanos, Theodoros

Citation:

Citation: Hobbs, Billy; Limmer, Noor; Ossa, Felipe; Knupling, Ella; Lenton, Samuel; Fodera, Vito; Kalverda, Arnout; Karamanos, Theodoros. "A low-complexity linker as a driver of intra- and intermolecular interactions in DNAJB chaperones" Nat. Commun. 16, 5070-5070 (2025).
PubMed: 40450039

Assembly members:

Assembly members:
entity_1, polymer, 111 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MGKDYYQTLGLARGASDEEI KRAYRRQALRYHPDKNKEPG AEEKFKEIAEAYDVLSDPRK REIFDRYGEEGLKGSGPSGG SGGGANGTSFSLTFHGDPHA MFAEFFGGRNP

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
15N chemical shifts	98
1H chemical shifts	98

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	DNAJB1 Y92L	1

Entities:

Entity 1, DNAJB1 Y92L 111 residues - Formula weight is not available

1

MET

GLY

LYS

ASP

TYR

GLN

THR

LEU

GLY

2

LEU

ALA

ARG

GLY

ALA

SER

ASP

GLU

ILE

3

LYS

ARG

ALA

TYR

ARG

GLN

ALA

LEU

ARG

4

TYR

HIS

PRO

ASP

LYS

ASN

LYS

GLU

PRO

GLY

5

ALA

GLU

LYS

PHE

LYS

GLU

ILE

ALA

GLU

6

ALA

TYR

ASP

VAL

LEU

SER

ASP

PRO

ARG

LYS

7

ARG

GLU

ILE

PHE

ASP

ARG

TYR

GLY

GLU

8

GLY

LEU

LYS

GLY

SER

GLY

PRO

SER

GLY

9

SER

GLY

ALA

ASN

GLY

THR

SER

PHE

10

SER

LEU

THR

PHE

HIS

GLY

ASP

PRO

HIS

ALA

11

MET

PHE

ALA

GLU

PHE

GLY

ARG

ASN

12

PRO

Samples:

sample_1: DNAJB1 Y92L, [U-100% 15N], 200 uM; sodium chloride 50 mM; sodium phosphate 20 mM; sodium azide 0.02 % w/v; MES 5 mM

sample_conditions_1: ionic strength: 75 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe - processing

CcpNMR - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 600 MHz

BMRB	50167
PDB	6Z5N