BMRB Entry 52736
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52736
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Hobbs, Billy; Limmer, Noor; Ossa, Felipe; Knupling, Ella; Lenton, Samuel; Fodera, Vito; Kalverda, Arnout; Karamanos, Theordoros. "A low-complexity linker as a driver of intra- and intermolecular interactions in DNAJB chaperones" Nat. Commun. 16, 5070-5070 (2025).
PubMed: 40450039
Assembly members:
entity_1, polymer, 96 residues, 11028.29 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-15b
Entity Sequences (FASTA):
entity_1: MVDYYEVLGVQRHASPEDIK
KAYRKLALKWHPDKNPENKE
EAERKFKQVAEAYEVLSDAK
KRDIYDKYGKEGLNGGGGGG
SHFDSPFEFGFTFRNP
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 254 |
| 15N chemical shifts | 80 |
| 1H chemical shifts | 80 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | DNAJB6 JD-GF | 1 |
Entities:
Entity 1, DNAJB6 JD-GF 96 residues - 11028.29 Da.
| 1 | MET | VAL | ASP | TYR | TYR | GLU | VAL | LEU | GLY | VAL | ||||
| 2 | GLN | ARG | HIS | ALA | SER | PRO | GLU | ASP | ILE | LYS | ||||
| 3 | LYS | ALA | TYR | ARG | LYS | LEU | ALA | LEU | LYS | TRP | ||||
| 4 | HIS | PRO | ASP | LYS | ASN | PRO | GLU | ASN | LYS | GLU | ||||
| 5 | GLU | ALA | GLU | ARG | LYS | PHE | LYS | GLN | VAL | ALA | ||||
| 6 | GLU | ALA | TYR | GLU | VAL | LEU | SER | ASP | ALA | LYS | ||||
| 7 | LYS | ARG | ASP | ILE | TYR | ASP | LYS | TYR | GLY | LYS | ||||
| 8 | GLU | GLY | LEU | ASN | GLY | GLY | GLY | GLY | GLY | GLY | ||||
| 9 | SER | HIS | PHE | ASP | SER | PRO | PHE | GLU | PHE | GLY | ||||
| 10 | PHE | THR | PHE | ARG | ASN | PRO |
Samples:
sample_1: DNAJB6 JD-GF, [U-13C; U-15N], 200 uM; sodium chloride 50 mM; sodium phosphate 20 mM; sodium azide 0.02 % w/v; MES 5 mM
sample_conditions_1: ionic strength: 75 mM; pH: 7; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACO | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe - processing
CcpNMR v2.4 - data analysis, peak picking
PINE - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks