BMRB Entry 52732

Name: GB1 Histag Atg12_42-62
Published: 2025-09-05

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52732

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

GB1 Histag Atg12_42-62

Deposition date:

2024-11-29

Original release date:

2025-09-05

Authors:

Fujioka, Yuko; Tsuji, Takuma; Katani, Tetsuya; Kumeta, Hiroyuki; Fujimoto, Toyoshi; Nakatogawa, Hitoshi; Noda, Nobuo

Citation:

Citation: Fujioka, Yuko; Tsuji, Takuma; Katani, Tetsuya; Kumeta, Hiroyuki; Fujimoto, Toyoshi; Nakatogawa, Hitoshi; Noda, Nobuo. "Phase separation promotes Atg8 lipidation for autophagy progression" Nat. Struct. Mol. Biol. ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 91 residues, Formula weight is not available

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGBHPS

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MEYKLILNGKTLKGETTTEA VDAATAEKVFKQYANDNGVD GEWTYDDATKTFTVTEHSHH HHHHGSSGSSTVQNRLELFS RRLSQLGLASD

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	230
15N chemical shifts	78
1H chemical shifts	300

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	GB1H6 Atg12_42-62	1

Entities:

Entity 1, GB1H6 Atg12_42-62 91 residues - Formula weight is not available

1

MET

GLU

TYR

LYS

LEU

ILE

LEU

ASN

GLY

LYS

2

THR

LEU

LYS

GLY

GLU

THR

GLU

ALA

3

VAL

ASP

ALA

THR

ALA

GLU

LYS

VAL

PHE

4

LYS

GLN

TYR

ALA

ASN

ASP

ASN

GLY

VAL

ASP

5

GLY

GLU

TRP

THR

TYR

ASP

ALA

THR

LYS

6

THR

PHE

THR

VAL

THR

GLU

HIS

SER

HIS

7

HIS

GLY

SER

GLY

SER

8

THR

VAL

GLN

ASN

ARG

LEU

GLU

LEU

PHE

SER

9

ARG

LEU

SER

GLN

LEU

GLY

LEU

ALA

SER

10

ASP

Samples:

sample_1: GB1H6 Atg12_42-62, [U-99% 13C; U-99% 15N], 1 mM; D2O, [U-2H], 10%; HEPES 20 mM; sodium chloride 150 mM

sample_conditions_1: ionic strength: 0.17 M; pH: 7.0; pressure: 1 atm; temperature: 298.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D 15N-separated NOESY	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v4.1.4 - collection

SPARKY v3.113 - chemical shift assignment, peak picking

NMR spectrometers:

Bruker AVANCE NEO 800 MHz
Bruker AVANCE III HD 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks