BMRB Entry 52667

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52667

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Title:
Backbone 1H, 13C and 15N Chemical Shift Assignments and Relaxation Parameters for USP14-USP domain
Deposition date:
2024-10-26
Original release date:
2025-04-07
Authors:
Salomonsson, Johannes; Sjostrand, Linda; Eskilson, Arvid; Derbyshire, Dean; D'Arcy, Padraig; Sunnerhagen, Maria; Ahlner, Alexandra
Citation:

Citation: Salomonsson, Johannes; Sjostrand, Linda; Eskilson, Arvid; Derbyshire, Dean; D'Arcy, Padraig; Sunnerhagen, Maria; Ahlner, Alexandra. "Dynamic networks connect the USP14 active site region with the proteasome interaction surface"  Protein Sci. 34, e70077-e70077 (2025).
PubMed: 40095364

Assembly members:

Assembly members:
entity_1, polymer, 494 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pNIC-28-Bsa4

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MPLYSVTVKWGKEKFEGVEL NTDEPPMVFKAQLFALTGVQ PARQKVMVKGGTLKDDDWGN IKIKNGMTLLMMGSADALPE EPSAKTVFVEDMTEEQLASA MELPCGLTNLGNTCYMNATV QCIRSVPELKDALKRYAGAL RASGEMASAQYITAALRDLF DSMDKTSSSIPPIILLQFLH MAFPQFAEKGEQGQYLQQDA NECWIQMMRVLQQKLEAIED DSVKETDSSSASAATPSKKK SLIDQFFGVEFETTMKCTES EEEEVTKGKENQLQLSCFIN QEVKYLFTGLKLRLQEEITK QSPTLQRNALYIKSSKISRL PAYLTIQMVRFFYKEKESVN AKVLKDVKFPLMLDMYELCT PELQEKMVSFRSKFKDLEDK KVNQQPNTSDKKSSPQKEVK YEPFSFADDIGSNNCGYYDL QAVLTHQGRSSSSGHYVSWV KRKQDEWIKFDDDKVSIVTP EDILRLSGGGDWHIAYVLLY GPRRVEIMEEESEQ

Data sets:
Data typeCount
13C chemical shifts1010
15N chemical shifts311
1H chemical shifts311
T1 relaxation values538
T2 relaxation values526
heteronuclear NOE values280

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1USP14-USP monomer1

Entities:

Entity 1, USP14-USP monomer 494 residues - Formula weight is not available

1   METPROLEUTYRSERVALTHRVALLYSTRP
2   GLYLYSGLULYSPHEGLUGLYVALGLULEU
3   ASNTHRASPGLUPROPROMETVALPHELYS
4   ALAGLNLEUPHEALALEUTHRGLYVALGLN
5   PROALAARGGLNLYSVALMETVALLYSGLY
6   GLYTHRLEULYSASPASPASPTRPGLYASN
7   ILELYSILELYSASNGLYMETTHRLEULEU
8   METMETGLYSERALAASPALALEUPROGLU
9   GLUPROSERALALYSTHRVALPHEVALGLU
10   ASPMETTHRGLUGLUGLNLEUALASERALA
11   METGLULEUPROCYSGLYLEUTHRASNLEU
12   GLYASNTHRCYSTYRMETASNALATHRVAL
13   GLNCYSILEARGSERVALPROGLULEULYS
14   ASPALALEULYSARGTYRALAGLYALALEU
15   ARGALASERGLYGLUMETALASERALAGLN
16   TYRILETHRALAALALEUARGASPLEUPHE
17   ASPSERMETASPLYSTHRSERSERSERILE
18   PROPROILEILELEULEUGLNPHELEUHIS
19   METALAPHEPROGLNPHEALAGLULYSGLY
20   GLUGLNGLYGLNTYRLEUGLNGLNASPALA
21   ASNGLUCYSTRPILEGLNMETMETARGVAL
22   LEUGLNGLNLYSLEUGLUALAILEGLUASP
23   ASPSERVALLYSGLUTHRASPSERSERSER
24   ALASERALAALATHRPROSERLYSLYSLYS
25   SERLEUILEASPGLNPHEPHEGLYVALGLU
26   PHEGLUTHRTHRMETLYSCYSTHRGLUSER
27   GLUGLUGLUGLUVALTHRLYSGLYLYSGLU
28   ASNGLNLEUGLNLEUSERCYSPHEILEASN
29   GLNGLUVALLYSTYRLEUPHETHRGLYLEU
30   LYSLEUARGLEUGLNGLUGLUILETHRLYS
31   GLNSERPROTHRLEUGLNARGASNALALEU
32   TYRILELYSSERSERLYSILESERARGLEU
33   PROALATYRLEUTHRILEGLNMETVALARG
34   PHEPHETYRLYSGLULYSGLUSERVALASN
35   ALALYSVALLEULYSASPVALLYSPHEPRO
36   LEUMETLEUASPMETTYRGLULEUCYSTHR
37   PROGLULEUGLNGLULYSMETVALSERPHE
38   ARGSERLYSPHELYSASPLEUGLUASPLYS
39   LYSVALASNGLNGLNPROASNTHRSERASP
40   LYSLYSSERSERPROGLNLYSGLUVALLYS
41   TYRGLUPROPHESERPHEALAASPASPILE
42   GLYSERASNASNCYSGLYTYRTYRASPLEU
43   GLNALAVALLEUTHRHISGLNGLYARGSER
44   SERSERSERGLYHISTYRVALSERTRPVAL
45   LYSARGLYSGLNASPGLUTRPILELYSPHE
46   ASPASPASPLYSVALSERILEVALTHRPRO
47   GLUASPILELEUARGLEUSERGLYGLYGLY
48   ASPTRPHISILEALATYRVALLEULEUTYR
49   GLYPROARGARGVALGLUILEMETGLUGLU
50   GLUSERGLUGLN

Samples:

sample_1: USP14_91-494, [U-99% 13C; U-99% 15N, U-99% 2H], 0.47 mM; HEPES 20 mM; sodium chloride 100 mM; TCEP 0.5 mM; sodium azide 0.02 mM

sample_2: USP14_99-494, [U-99% 15N, U-99% 2H], 0.89 mM; HEPES 20 mM; sodium chloride 100 mM; TCEP 0.5 mM; sodium azide 0.02 mM

sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 303.15 K

sample_conditions_2: pH: 7.5; pressure: 1 atm; temperature: 298.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSYsample_1isotropicsample_conditions_1
3D HNCA TROSYsample_1isotropicsample_conditions_1
3D HN(CO)CA TROSYsample_1isotropicsample_conditions_1
3D HNCACB TROSYsample_1isotropicsample_conditions_1
3D HN(CO)CACB TROSYsample_1isotropicsample_conditions_1
3D HNCO TROSYsample_1isotropicsample_conditions_1
3D HN(CA)CO TROSYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY TROSYsample_1isotropicsample_conditions_1
2D 1H-15N TROSYsample_2isotropicsample_conditions_2
T1/R1 relaxationsample_2isotropicsample_conditions_2
T2/R2 relaxationsample_2isotropicsample_conditions_2
15N-(1H) NOEsample_2isotropicsample_conditions_2
T1/R1 relaxationsample_2isotropicsample_conditions_2
T2/R2 relaxationsample_2isotropicsample_conditions_2

Software:

MddNMR - processing

NMRPipe - processing

CYANA - chemical shift assignment, peak picking

COMPASS - chemical shift assignment

NMRFAM-SPARKY - chemical shift assignment, peak picking

PINT - data analysis

NMR spectrometers:

  • Bruker AVANCE III HD 800 MHz
  • Bruker AVANCE III HD 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks