BMRB Entry 52457

Name: p53TAD2 bound to FOXO4-DRI
Published: 2025-01-31

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52457

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

p53TAD2 bound to FOXO4-DRI

Deposition date:

2024-05-13

Original release date:

2025-01-31

Authors:

Bourgeois, Benjamin; Spreitzer, Emil; Platero-Rochart, Daniel; Zhou, Qishun; Usluer, Sinem; de Keizer, Peter; Burgering, Boudewijn; Sanchez-Murcia, Pedro; Madl, Tobias

Citation:

Citation: Bourgeois, Benjamin; Spreitzer, Emil; Platero-Rochart, Daniel; Zhou, Qishun; Usluer, Sinem; de Keizer, Peter; Burgering, Boudewijn; Sanchez-Murcia, Pedro; Madl, Tobias. "The Disordered p53 Transactivation Domain is the Target of FOXO4 and the Senolytic Compound FOXO4-DRI" Nat. Commun. ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 21 residues, Formula weight is not available
entity_2, polymer, 46 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM11-ZZ-His6

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SQAMDDLMLSPDDIEQWFTE D
entity_2: LTLRKEPASEIAQSILEAYS QNGWANRRSGGKRPPPRRRQ RRKKRG

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	63
15N chemical shifts	19
1H chemical shifts	128

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	p53TAD2	1
2	FOXO4-LRI	2

Entities:

Entity 1, p53TAD2 21 residues - Formula weight is not available

1

SER

GLN

ALA

MET

ASP

LEU

MET

LEU

SER

2

PRO

ASP

ILE

GLU

GLN

TRP

PHE

THR

GLU

3

ASP

Entity 2, FOXO4-LRI 46 residues - Formula weight is not available

1

LEU

THR

LEU

ARG

LYS

GLU

PRO

ALA

SER

GLU

2

ILE

ALA

GLN

SER

ILE

LEU

GLU

ALA

TYR

SER

3

GLN

ASN

GLY

TRP

ALA

ASN

ARG

SER

GLY

4

GLY

LYS

ARG

PRO

ARG

GLN

5

ARG

LYS

ARG

GLY

Samples:

sample_1: p53TAD2, [U-99% 13C; U-99% 15N], 1160 uM; FOXO4-DRI 1160 uM; D2O 10%; DTT 2.5 mM

sample_conditions_1: ionic strength: 0 M; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR v2.5 - chemical shift assignment

NMR spectrometers:

Bruker AVANCE NEO 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks