BMRB Entry 52442

Name: RGS domain of human RGS10
Published: 2025-06-20

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52442

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

RGS domain of human RGS10

Deposition date:

2024-05-03

Original release date:

2025-06-20

Authors:

Urbauer, Jeffrey; Hooks, Shelley; Bieber Urbauer, Ramona; Tope Niedermaier, Cynthia; Ramirez Quintero, Karen

Citation:

Citation: Ramirez Quintero, Karen; Tope Niedermaier, Cynthia; Hooks, Shelley; Bieber Urbauer, Ramona; Urbauer, Jeffrey. "RGS domain of human RGS10" .

Assembly members:

Assembly members:
entity_1, polymer, 135 residues, 16140 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-19

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SLKSTAKWAASLENLLEDPE GVKRFREFLKKEFSEENVLF WLACEDFKKMQDKTQMQEKA KEIYMTFLSSKASSQVNVEG QSRLNEKILEEPHPLMFQKL QDQIFNLMKYDSYSRFLKSD LFLKHKRTEEEEEDL

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	598
15N chemical shifts	144
1H chemical shifts	953

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RGS domain of human RGS10	1

Entities:

Entity 1, RGS domain of human RGS10 135 residues - 16140 Da.

1

SER

LEU

LYS

SER

THR

ALA

LYS

TRP

ALA

2

SER

LEU

GLU

ASN

LEU

GLU

ASP

PRO

GLU

3

GLY

VAL

LYS

ARG

PHE

ARG

GLU

PHE

LEU

LYS

4

LYS

GLU

PHE

SER

GLU

ASN

VAL

LEU

PHE

5

TRP

LEU

ALA

CYS

GLU

ASP

PHE

LYS

MET

6

GLN

ASP

LYS

THR

GLN

MET

GLN

GLU

LYS

ALA

7

LYS

GLU

ILE

TYR

MET

THR

PHE

LEU

SER

8

LYS

ALA

SER

GLN

VAL

ASN

VAL

GLU

GLY

9

GLN

SER

ARG

LEU

ASN

GLU

LYS

ILE

LEU

GLU

10

GLU

PRO

HIS

PRO

LEU

MET

PHE

GLN

LYS

LEU

11

GLN

ASP

GLN

ILE

PHE

ASN

LEU

MET

LYS

TYR

12

ASP

SER

TYR

SER

ARG

PHE

LEU

LYS

SER

ASP

13

LEU

PHE

LEU

LYS

HIS

LYS

ARG

THR

GLU

14

GLU

ASP

LEU

Samples:

sample_1: RGS domain of human RGS10, [U-99% 13C; U-99% 15N], 0.5 ± 0.05 mM; imidazole, [U-98% 2H], 1 ± 0.1 mM; KCl 1 ± 0.1 mM; CaCl2 10 ± 0.05 mM; TCEP 0.1 ± 0.01 mM; sodium azide 0.02 ± 0.002 %; D2O, [U-2H], 5 ± 0.5 %

sample_2: RGS domain of human RGS10, [U-10% 13C; U-99% 15N], 0.5 ± 0.05 mM; imidazole, [U-98% 2H], 1 ± 0.1 mM; KCl 1 ± 0.1 mM; CaCl2 10 ± 0.05 mM; TCEP 0.1 ± 0.01 mM; sodium azide 0.02 ± 0.002 %; D2O, [U-2H], 5 ± 0.5 %

sample_conditions_1: ionic strength: 41 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-13C HSQC aliphatic	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D HCCH-COSY	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH-TOCSY	sample_1	isotropic	sample_conditions_1
3D C(CO)NH-TOCSY	sample_1	isotropic	sample_conditions_1
2D (HB)CB(CGCD)HD	sample_1	isotropic	sample_conditions_1
3D 1H-TOCSY-relayed ct-[13C,1H]-HMQC	sample_1	isotropic	sample_conditions_1
3D 15N-separated NOESY	sample_1	isotropic	sample_conditions_1
3D 13C-separated NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_1	isotropic	sample_conditions_1
3D 13C-separated NOESY aromatic	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_2	isotropic	sample_conditions_1

Software:

VNMRj v4.2 - collection

Felix NMR v2007 - chemical shift assignment, chemical shift calculation, data analysis, peak picking, processing

NMRFAM-SPARKY v1.470 - chemical shift assignment

TALOS-N - chemical shift calculation

NMR spectrometers:

Agilent VNMRS 600 MHz