BMRB Entry 5203

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5203
MolProbity Validation Chart

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Title:
1H, 13C, 15N chemical shift assignments for cysteine-rich domain of KSR
Deposition date:
2001-11-06
Original release date:
2002-05-07
Authors:
Zhou, Ming; Horita, David; Waugh, David; Byrd, R.; Morrison, Deborah
Citation:

Citation: Zhou, Ming; Horita, David; Waugh, David; Byrd, R.; Morrison, Deborah. "Solution Structure and Functional Analysis of the Cysteine-rich C1 Domain of Kinase Suppressor of Ras (KSR) "  J. Mol. Biol. 315, 435-446 (2002).
PubMed: 11786023

Assembly members:

Assembly members:
Kinase suppressor of Ras, polymer, 49 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Kinase suppressor of Ras: GSVTHRFSTKSWLSQVCNVC QKSMIFGVKCKHCRLKCHNK CTKEAPACR

Data sets:
Data typeCount
1H chemical shifts270
13C chemical shifts138
15N chemical shifts44

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1cysteine-rich C1 domain of KSR1
2Zinc(II) ion, I2
3Zinc(II) ion, II2

Entities:

Entity 1, cysteine-rich C1 domain of KSR 49 residues - Formula weight is not available

1   GLYSERVALTHRHISARGPHESERTHRLYS
2   SERTRPLEUSERGLNVALCYSASNVALCYS
3   GLNLYSSERMETILEPHEGLYVALLYSCYS
4   LYSHISCYSARGLEULYSCYSHISASNLYS
5   CYSTHRLYSGLUALAPROALACYSARG

Entity 2, Zinc(II) ion, I - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: Kinase suppressor of Ras, [U-15N; U-13C], 1.0 mM; ZINC (II) ION 2.0 mM

Ex-cond_1: pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HSQCsample_1not availableEx-cond_1
NOESY-HSQCsample_1not availableEx-cond_1
CBCACONHsample_1not availableEx-cond_1
HNCACBsample_1not availableEx-cond_1
TOCSY-HSQCsample_1not availableEx-cond_1
3dCN-NOESYsample_1not availableEx-cond_1
HCCH TOCSYsample_1not availableEx-cond_1

Software:

Ansig v3.3 -

NMR spectrometers:

  • Varian UnityPlus 500 MHz
  • Varian UnityPlus 600 MHz

Related Database Links:

SWISS-PROT Q61097
REF NP_038599
GenBank EDL15602 EDL15601 ABK42251 AAI68386 AAC52382
EMBL CAI24047 CAI24046 CAA57288
DBJ BAE23398
PDB

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