BMRB Entry 51866

Name: Assignments for fibrillar Cysteine to Serine Double Mutant of Human Islet Amyloid Polypeptide (hIAPP_C2S/C7S)
Published: 2024-10-23

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51866

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Assignments for fibrillar Cysteine to Serine Double Mutant of Human Islet Amyloid Polypeptide (hIAPP_C2S/C7S)

Deposition date:

2023-03-03

Original release date:

2024-10-23

Authors:

Suladze, Saba; Sankar, Riddhiman; Rodriguez Camargo, Diana; Reif, Bernd

Citation:

Citation: Suladze, Saba; Sustay Martinez, Christian; Rodriguez Camargo, Diana; Engler, Jonas; Rodina, Natalia; Sarkar, Riddhiman; Zacharias, Martin; Reif, Bernd. "Structural Insights into Seeding Mechanisms of hIAPP Fibril Formation" J. Am. Chem. Soc. 146, 13783-13796 (2024).
PubMed: 38723619

Assembly members:

Assembly members:
entity_1, polymer, 37 residues, 3874.20 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pTXB1EEI

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: KSNTATSATQRLANFLVHSS NNFGAILSSTNVGSNTY

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	96
15N chemical shifts	32
1H chemical shifts	32

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	hIAPP_C2S	1

Entities:

Entity 1, hIAPP_C2S 37 residues - 3874.20 Da.

1

LYS

SER

ASN

THR

ALA

THR

SER

ALA

THR

GLN

2

ARG

LEU

ALA

ASN

PHE

LEU

VAL

HIS

SER

3

ASN

PHE

GLY

ALA

ILE

LEU

SER

THR

4

ASN

VAL

GLY

SER

ASN

THR

TYR

Samples:

sample_1: Fibrils of hIAPP_C2S/C7S, [U-100% 15N], 92.5 mM

sample_conditions_1: ionic strength: 0.030 M; pH: 5.3; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 15N-1H "CP-HSQC"	sample_1	isotropic	sample_conditions_1
3D (H)CANH	sample_1	isotropic	sample_conditions_1
3D (H)(CO)CA(CO)NH	sample_1	isotropic	sample_conditions_1
3D (H)(CA)CB(CA)NH	sample_1	isotropic	sample_conditions_1
3D (H)CONH	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR v2.4.2 - chemical shift assignment

NMR spectrometers:

Bruker Avance 850 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks