BMRB Entry 5163
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5163
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Citation: Lamosa, P.; Brennan, L.; Vis, H.; Turner, D.; Santos, H.. "NMR Structure of Desulfovibrio gigas Rubredoxin: a Model for studying Protein
Stabilization by Compatible Solutes" Extremophiles 5, 303-311 (2001).
PubMed: 11699644
Assembly members:
RUBREDOXIN, polymer, 52 residues, 65.409 Da.
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Desulfovibrio gigas Taxonomy ID: 879 Superkingdom: Bacteria Kingdom: not available Genus/species: Desulfovibrio gigas
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
RUBREDOXIN: MDIYVCTVCGYEYDPAKGDP
DSGIKPGTKFEDLPDDWACP
VCGASKDAFEKQ
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 282 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | rubredoxin | 1 |
| 2 | zinc ion | 2 |
Entities:
Entity 1, rubredoxin 52 residues - 65.409 Da.
| 1 | MET | ASP | ILE | TYR | VAL | CYS | THR | VAL | CYS | GLY | ||||
| 2 | TYR | GLU | TYR | ASP | PRO | ALA | LYS | GLY | ASP | PRO | ||||
| 3 | ASP | SER | GLY | ILE | LYS | PRO | GLY | THR | LYS | PHE | ||||
| 4 | GLU | ASP | LEU | PRO | ASP | ASP | TRP | ALA | CYS | PRO | ||||
| 5 | VAL | CYS | GLY | ALA | SER | LYS | ASP | ALA | PHE | GLU | ||||
| 6 | LYS | GLN |
Entity 2, zinc ion - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: RUBREDOXIN 2.5 mM; ZINC (II) ION 2.5 mM
sample_cond_1: pH: 7.6; temperature: 308 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H NOESY | sample_1 | not available | sample_cond_1 |
| 2D 1H TOCSY | sample_1 | not available | sample_cond_1 |
| 2D 1H COSY | sample_1 | not available | sample_cond_1 |
Software:
No software information available
NMR spectrometers:
- BRUKER DRX 500 MHz