BMRB Entry 50768

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50768

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Title:
1H and 13C chemical-shift assignments of the isoleucine, leucine and valine methyl groups of Bombyx Mori Vasa
Deposition date:
2021-02-17
Original release date:
2024-10-15
Authors:
Codutti, Luca; Nesme, Leo; Kirkpatrick, John; Carlomagno, Teresa
Citation:

Citation: Codutti, Luca; Kirkpatrick, John; zur Lage, Suzanne; Carlomagno, Teresa. "Long-range conformational changes in the nucleotide-bound states of the DEAD-box helicase Vasa"  Biophys. J. ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 438 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: domestic silkworm   Taxonomy ID: 7091   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bombyx mori

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETM11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GAMDPAEFVTYVPPEPTNDE TEIFSSTISSGINFDKFDHI AVKVSGENPPRPIESFETAN LRKYVLDNVLKAGYRKPTPI QKNAIPIIMSGRDLMGCAQT GSGKTAAFLVPIINMLLQDP KDLISENGCAQPQVIIVSPT RELTLQIFNEARKFSYGSVL KVAVAYGGTAVRHQGDNIAR GCHILVATPGRLHDFVERNR VSFGSVRFVVLDEADRMLDM GFMPSIEKMMLHPTMVETTK RQTLMFSATFPEDIQHLAGR FLNNYLFVAVGIVGGASTDV EQIFIEVTKYEKRNSLKQLI EENDGKRILVFVETKRNADF IAAMLSEQQLLTSSIHGDRM QREREEALQNFKSGKHCILV ATAVAARGLDIKNVDIVVNY DLPKSIDEYVHRIGRTGRVG NRGKAVSFYDSDQDLALVAD LSKILRQADQSVPDFLKG

Data sets:
Data typeCount
13C chemical shifts150
1H chemical shifts450

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1flVasa1

Entities:

Entity 1, flVasa 438 residues - Formula weight is not available

The first residue of the native Vasa sequence is residue 9 in the sequence above, which corresponds to residue 135 in the native Vasa sequence; hence residue 1 of the sequence above should be numbered as residue 127 (i.e. the first 8 residues are cloning artifacts).

1   GLYALAMETASPPROALAGLUPHEVALTHR
2   TYRVALPROPROGLUPROTHRASNASPGLU
3   THRGLUILEPHESERSERTHRILESERSER
4   GLYILEASNPHEASPLYSPHEASPHISILE
5   ALAVALLYSVALSERGLYGLUASNPROPRO
6   ARGPROILEGLUSERPHEGLUTHRALAASN
7   LEUARGLYSTYRVALLEUASPASNVALLEU
8   LYSALAGLYTYRARGLYSPROTHRPROILE
9   GLNLYSASNALAILEPROILEILEMETSER
10   GLYARGASPLEUMETGLYCYSALAGLNTHR
11   GLYSERGLYLYSTHRALAALAPHELEUVAL
12   PROILEILEASNMETLEULEUGLNASPPRO
13   LYSASPLEUILESERGLUASNGLYCYSALA
14   GLNPROGLNVALILEILEVALSERPROTHR
15   ARGGLULEUTHRLEUGLNILEPHEASNGLU
16   ALAARGLYSPHESERTYRGLYSERVALLEU
17   LYSVALALAVALALATYRGLYGLYTHRALA
18   VALARGHISGLNGLYASPASNILEALAARG
19   GLYCYSHISILELEUVALALATHRPROGLY
20   ARGLEUHISASPPHEVALGLUARGASNARG
21   VALSERPHEGLYSERVALARGPHEVALVAL
22   LEUASPGLUALAASPARGMETLEUASPMET
23   GLYPHEMETPROSERILEGLULYSMETMET
24   LEUHISPROTHRMETVALGLUTHRTHRLYS
25   ARGGLNTHRLEUMETPHESERALATHRPHE
26   PROGLUASPILEGLNHISLEUALAGLYARG
27   PHELEUASNASNTYRLEUPHEVALALAVAL
28   GLYILEVALGLYGLYALASERTHRASPVAL
29   GLUGLNILEPHEILEGLUVALTHRLYSTYR
30   GLULYSARGASNSERLEULYSGLNLEUILE
31   GLUGLUASNASPGLYLYSARGILELEUVAL
32   PHEVALGLUTHRLYSARGASNALAASPPHE
33   ILEALAALAMETLEUSERGLUGLNGLNLEU
34   LEUTHRSERSERILEHISGLYASPARGMET
35   GLNARGGLUARGGLUGLUALALEUGLNASN
36   PHELYSSERGLYLYSHISCYSILELEUVAL
37   ALATHRALAVALALAALAARGGLYLEUASP
38   ILELYSASNVALASPILEVALVALASNTYR
39   ASPLEUPROLYSSERILEASPGLUTYRVAL
40   HISARGILEGLYARGTHRGLYARGVALGLY
41   ASNARGGLYLYSALAVALSERPHETYRASP
42   SERASPGLNASPLEUALALEUVALALAASP
43   LEUSERLYSILELEUARGGLNALAASPGLN
44   SERVALPROASPPHELEULYSGLY

Samples:

sample_1: flVasa, [U-13C; U-15N; U-2H; 99% 1HD-Ile,Leu; 99% 1HG-Val], 0.5 ± 0.1 mM; TRIS 50 mM; sodium chloride 350 mM; arginine 100 mM; glutamic acid 100 mM; magnesium chloride 1 mM; TCEP 1 mM; sodium azide 0.01%

sample_conditions_1: pH: 8.0; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HMQCsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.2 - collection, processing

NMRPipe v10.1 - processing

CcpNMR v2.4 - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker AVANCE III HD 850 MHz

Related Database Links:

NCBI NP_001037347.1