BMRB Entry 4821

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4821

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Title:
Assignment of 1H, 13C, and 15N resonances to the sensory domain of membraneous two-component fumarate sensor (histidine protein kinase) DcuS of Escherichia coli
Deposition date:
2000-09-07
Original release date:
2000-11-29
Authors:
Parac, Tatjana; Coligaev, Boris; Peti, Wolfgang; Zientz, Evelyne; Unden, Gottfried; Griesinger, Christian
Citation:

Citation: Parac, Tatjana; Coligaev, Boris; Zientz, Evelyne; Unden, Gottfried; Peti, Wolfgang; Griesinger, Christian. "Letter to the Editor: Assignment of 1H, 13C, and 15N resonances to the sensory domain of membraneous two-component fumarate sensor (histidine protein kinase) DcuS of Escherichia coli"  J. Biomol. NMR 19, 91-92 (2001).

Assembly members:

Assembly members:
DcuS, polymer, 157 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E.coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DcuS: MGSSHHHHHHSSGLVPRGSH MSDMTRDGLANKALAVARTL ADSPEIRQGLQKKPQESGIQ AIAEAVRKRNDLLFIVVTDM QSLRYSHPEAQRIGQPFKGD DILKALNGEENVAINRGFLA QALRVFTPIYDENHKQIGVV AIGLELSRVTQQINDSR

Data sets:
Data typeCount
1H chemical shifts950
13C chemical shifts559
15N chemical shifts140

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DcuS1

Entities:

Entity 1, DcuS 157 residues - Formula weight is not available

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METSERASPMETTHRARGASPGLYLEUALA
4   ASNLYSALALEUALAVALALAARGTHRLEU
5   ALAASPSERPROGLUILEARGGLNGLYLEU
6   GLNLYSLYSPROGLNGLUSERGLYILEGLN
7   ALAILEALAGLUALAVALARGLYSARGASN
8   ASPLEULEUPHEILEVALVALTHRASPMET
9   GLNSERLEUARGTYRSERHISPROGLUALA
10   GLNARGILEGLYGLNPROPHELYSGLYASP
11   ASPILELEULYSALALEUASNGLYGLUGLU
12   ASNVALALAILEASNARGGLYPHELEUALA
13   GLNALALEUARGVALPHETHRPROILETYR
14   ASPGLUASNHISLYSGLNILEGLYVALVAL
15   ALAILEGLYLEUGLULEUSERARGVALTHR
16   GLNGLNILEASNASPSERARG

Samples:

sample_1: DcuS, [U-99% 13C; U-99% 15N], 1 mM; H2O 90%; D2O 10%; sodium phosphate 50 mM; NaCl 200 mM; Glycine 50 mM; Pefabloc SC 50 pM; NaN3 0.01%

Cond1: pH: 6.5; temperature: 303 K; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
15N HSQCnot availablenot availablenot available
NHCOnot availablenot availablenot available
NHCOCACBnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CCCONHnot availablenot availablenot available
HCCH-COSYnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
HBHACONHnot availablenot availablenot available
15N NOESYHSQCnot availablenot availablenot available
15N HSQCNOESYHSQCnot availablenot availablenot available
13C NOESYHSQCnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DRX 800 MHz

Related Database Links:

PDB
DBJ BAB38530 BAE78127 BAG79947 BAI28380 BAI33564
EMBL CAP78597 CAR01100 CAR05782 CAR10963 CAR15772
GB AAA97025 AAC77086 AAG59324 AAN45523 AAN83553
REF NP_290758 NP_313134 NP_418549 NP_709816 NP_756979
SP P0AEC8 P0AEC9 P59340 P59341
AlphaFold P0AEC8 P0AEC9 P59340 P59341

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks