BMRB Entry 4457

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4457

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Sequence-specific assignments and partial unfolding of extracellular domains II and III of E-cadherin.

Deposition date:

1999-11-15

Original release date:

2000-03-17

Authors:

Alattia, Jean-Rene; Tong, Frances; Tong, Kit; Ikura, Mitsuhiko

Citation:

Citation: Alattia, Jean-Rene; Tong, Frances; Tong, Kit; Ikura, Mitsuhiko. "Letter to the Editor: Sequence-specific resonance assignments and partial unfolding of extracellular domains II and III of E-cadherin" J. Biomol. NMR 16, 181-182 (2000).

Assembly members:

Assembly members:
Epithelial Cadherin, polymer, 227 residues, 24355 Da.

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET 15b (modified)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Epithelial Cadherin: GSMTQEVFEGSVAEGAVPGT SVMKVSATDADDDVNTYNAA IAYTIVSQDPELPHKNMFTV NRDTGVISVLTSGLDRESYP TYTLVVQAADLQGEGLSTTA KAVITVKDINDNAPVFNPST YQGQVPENEVNARIATLKVT DDDAPNTPAWKAVYTVVNDP DQQFVVVTDPTTNDGILKTA KGLDFEAKQQYILHVRVENE EPFEGSLVPSTATVTVDVVD VNEAPIF

Data sets:

assigned_chemical_shifts
- curr_side_assign.16

Data type	Count
1H chemical shifts	332
13C chemical shifts	321
15N chemical shifts	167

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	E-cadherin domains 2-3	1

Entities:

Entity 1, E-cadherin domains 2-3 227 residues - 24355 Da.

1

GLY

SER

MET

THR

GLN

GLU

VAL

PHE

GLU

GLY

2

SER

VAL

ALA

GLU

GLY

ALA

VAL

PRO

GLY

THR

3

SER

VAL

MET

LYS

VAL

SER

ALA

THR

ASP

ALA

4

ASP

VAL

ASN

THR

TYR

ASN

ALA

5

ILE

ALA

TYR

THR

ILE

VAL

SER

GLN

ASP

PRO

6

GLU

LEU

PRO

HIS

LYS

ASN

MET

PHE

THR

VAL

7

ASN

ARG

ASP

THR

GLY

VAL

ILE

SER

VAL

LEU

8

THR

SER

GLY

LEU

ASP

ARG

GLU

SER

TYR

PRO

9

THR

TYR

THR

LEU

VAL

GLN

ALA

ASP

10

LEU

GLN

GLY

GLU

GLY

LEU

SER

THR

ALA

11

LYS

ALA

VAL

ILE

THR

VAL

LYS

ASP

ILE

ASN

12

ASP

ASN

ALA

PRO

VAL

PHE

ASN

PRO

SER

THR

13

TYR

GLN

GLY

GLN

VAL

PRO

GLU

ASN

GLU

VAL

14

ASN

ALA

ARG

ILE

ALA

THR

LEU

LYS

VAL

THR

15

ASP

ALA

PRO

ASN

THR

PRO

ALA

TRP

16

LYS

ALA

VAL

TYR

THR

VAL

ASN

ASP

PRO

17

ASP

GLN

PHE

VAL

THR

ASP

PRO

18

THR

ASN

ASP

GLY

ILE

LEU

LYS

THR

ALA

19

LYS

GLY

LEU

ASP

PHE

GLU

ALA

LYS

GLN

20

TYR

ILE

LEU

HIS

VAL

ARG

VAL

GLU

ASN

GLU

21

GLU

PRO

PHE

GLU

GLY

SER

LEU

VAL

PRO

SER

22

THR

ALA

THR

VAL

THR

VAL

ASP

VAL

ASP

23

VAL

ASN

GLU

ALA

PRO

ILE

PHE

Samples:

ec23_274c: Epithelial Cadherin 2.0 mM

cond_274c: pH: 7.4; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N NOESY	not available	not available	not available
1H-15N DIPSI	not available	not available	not available
HBCBCACONNH	not available	not available	not available
HNCACB	not available	not available	not available
HNCO	not available	not available	not available
HBCBCACOCAHA	not available	not available	not available
HCCH-TOCSY	not available	not available	not available
CCCTOCSY_NNH	not available	not available	not available
15N-13C NOESY	not available	not available	not available

Software:

nmrPipe - Data processing

nmrview v3.0b4 - Assignment

NMR spectrometers:

Varian Inova 500 MHz
Varian Inova 600 MHz

REF	NP_033994.1
SWISS-PROT	P09803
GenBank	EDL11373.1 AAH98501.1 EDL11372.1
PRF	1312359A
EMBL	CAA29488.1 CAA43292.1 CAA29645.1
PDB	1EDH