BMRB Entry 4348

Name: 1H, 15N and 13C Chemical Shift Assignments for NtrC-C-term-3Ala
Published: 1999-10-06

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4348

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 15N and 13C Chemical Shift Assignments for NtrC-C-term-3Ala

Deposition date:

1999-05-18

Original release date:

1999-10-06

Authors:

Pelton, Jeffrey; Kustu, Sydney; Wemmer, David

Citation:

Citation: Pelton, Jeffrey; Kustu, Sydney; Wemmer, David. "Solution Structure of the DNA-binding Domain of NtrC with Three Alanine Substitutions" J. Mol. Biol. ., .-..

Assembly members:

Assembly members:
Nitrogen regulatory protein C, polymer, 91 residues, 19962 Da.

Natural source:

Natural source: Common Name: Salmonella typhimurium Taxonomy ID: 602 Superkingdom: Eubacteria Kingdom: not available Genus/species: Salmonella typhimurium

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pJES1092

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Nitrogen regulatory protein C: MDLPGELFEASTPDSPSHLP PDSWATLLAQWADRALRSGH QNLLSEAQPELERTLLTTAL RHTQGHKQEAARLLGWGAAT LTAKLKELGME

Data sets:

assigned_chemical_shifts
- shift_NtrC-C-term-3Ala

Data type	Count
13C chemical shifts	281
15N chemical shifts	77
1H chemical shifts	430

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	NtrC-C-term-3Ala subunit 1	1
2	NtrC-C-term-3Ala subunit 2	1

Entities:

Entity 1, NtrC-C-term-3Ala subunit 1 91 residues - 19962 Da.

1

MET

ASP

LEU

PRO

GLY

GLU

LEU

PHE

GLU

ALA

2

SER

THR

PRO

ASP

SER

PRO

SER

HIS

LEU

PRO

3

PRO

ASP

SER

TRP

ALA

THR

LEU

ALA

GLN

4

TRP

ALA

ASP

ARG

ALA

LEU

ARG

SER

GLY

HIS

5

GLN

ASN

LEU

SER

GLU

ALA

GLN

PRO

GLU

6

LEU

GLU

ARG

THR

LEU

THR

ALA

LEU

7

ARG

HIS

THR

GLN

GLY

HIS

LYS

GLN

GLU

ALA

8

ALA

ARG

LEU

GLY

TRP

GLY

ALA

THR

9

LEU

THR

ALA

LYS

LEU

LYS

GLU

LEU

GLY

MET

10

GLU

Samples:

sample_1: Nitrogen regulatory protein C, [U-98% 13C; U-95% 15N], 0.5 – 1.5 mM; sodium_phosphate 20 mM; potassium_chloride 50 mM; EDTA 0.1 mM; sodium_azide 0.02 w/v %

Ex-cond_1: pH: 6.1; temperature: 308 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N NOESY-HSQC	not available	not available	not available
1H-15N TOCSY-HSQC	not available	not available	not available
HNCA	not available	not available	not available
HCACO	not available	not available	not available
HNCO	not available	not available	not available
HA(CO)NH	not available	not available	not available

Software:

No software information available

NMR spectrometers:

Bruker AMX 600 MHz
Bruker DRX 500 MHz