BMRB Entry 4189
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR4189
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
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Citation: Banci, L.; Bertini, I.; Huber, J.; Spyroulias, G.; Turano, P.. "Solution Structure of Reduced Horse Heart Cytochrome C" J. Biol. Inorg. Chem. 4, 21-31 (1999).
Assembly members:
cytochrome c, polymer, 104 residues, Formula weight is not available
HEC, non-polymer, 618.503 Da.
Natural source: Common Name: horse Taxonomy ID: 9796 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Equus caballus
Experimental source: Production method: .
Entity Sequences (FASTA):
cytochrome c: GDVEKGKKIFVQKCAQCHTV
EKGGKHKTGPNLHGLFGRKT
GQAPGFTYTDANKNKGITWK
EETLMEYLENPKKYIPGTKM
IFAGIKKKTEREDLIAYLKK
ATNE
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 709 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | hh cytc | 1 |
| 2 | heme | 2 |
Entities:
Entity 1, hh cytc 104 residues - Formula weight is not available
| 1 | GLY | ASP | VAL | GLU | LYS | GLY | LYS | LYS | ILE | PHE | ||||
| 2 | VAL | GLN | LYS | CYS | ALA | GLN | CYS | HIS | THR | VAL | ||||
| 3 | GLU | LYS | GLY | GLY | LYS | HIS | LYS | THR | GLY | PRO | ||||
| 4 | ASN | LEU | HIS | GLY | LEU | PHE | GLY | ARG | LYS | THR | ||||
| 5 | GLY | GLN | ALA | PRO | GLY | PHE | THR | TYR | THR | ASP | ||||
| 6 | ALA | ASN | LYS | ASN | LYS | GLY | ILE | THR | TRP | LYS | ||||
| 7 | GLU | GLU | THR | LEU | MET | GLU | TYR | LEU | GLU | ASN | ||||
| 8 | PRO | LYS | LYS | TYR | ILE | PRO | GLY | THR | LYS | MET | ||||
| 9 | ILE | PHE | ALA | GLY | ILE | LYS | LYS | LYS | THR | GLU | ||||
| 10 | ARG | GLU | ASP | LEU | ILE | ALA | TYR | LEU | LYS | LYS | ||||
| 11 | ALA | THR | ASN | GLU |
Entity 2, heme - C34 H34 Fe N4 O4 - 618.503 Da.
| 1 | HEC |
Samples:
sample_one: cytochrome c2.5 – 3.0 mM; phosphate 100 mM
sample_two: cytochrome c2.5 – 3.0 mM; phosphate 100 mM
sample_conditions_one: pH: 7.0; pressure: 1013 mbar; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| NOESY | sample_one | not available | sample_conditions_one |
| TOCSY | sample_one | not available | sample_conditions_one |
Software:
xwinnmr - Multiplication of raw data by a pure cosine-squared function Fourier Transform Polynomial baseline correction
XEASY - Volume integration of cross-peaks on 2D spectra
NMR spectrometers:
- BRUKER AVANCE 800 MHz
- BRUKER AVANCE 600 MHz