BMRB Entry 4161

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4161

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N Assignment of the DNA Binding Domain of Interferon Regulatory Factor-2

Deposition date:

1998-07-14

Original release date:

2000-03-21

Authors:

Furui, Junichi; Uegaki, Koichi; Yamazaki, Toshio; Shirakawa, Masahiro; Harada, Hisashi; Taniguchi, Tadatugu; Kyogoku, Yashimasa

Citation:

Citation: Furui, Junichi; Uegaki, Koichi; Yamazaki, Toshio; Shirakawa, Masahiro; Harada, Hisashi; Taniguchi, Tadatugu; Kyogoku, Yashimasa. "Solution structure of the IRF-2 DNA-binding domain: a novel subgroup of the winged helix-turn-helix family" Structure 6, 491-500 (1998).
PubMed: 9562558

Assembly members:

Assembly members:
IRF-2 DNA binding domain, polymer, 113 residues, Formula weight is not available

Natural source:

Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
IRF-2 DNA binding domain: PVERMRMRPWLEEQINSNTI PGLKWLNKEKKIFQIPWMHA ARHGWDVEKDAPLFRNWAIH TGKHQPGIDKPDPKTWKANF RCAMNSLPDIEEVKDRSIKK GNNAFRVYRMLPA

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_one

Data type	Count
13C chemical shifts	432
15N chemical shifts	118
1H chemical shifts	726

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	IRF-2(113)	1

Entities:

Entity 1, IRF-2(113) 113 residues - Formula weight is not available

1

PRO

VAL

GLU

ARG

MET

ARG

MET

ARG

PRO

TRP

2

LEU

GLU

GLN

ILE

ASN

SER

ASN

THR

ILE

3

PRO

GLY

LEU

LYS

TRP

LEU

ASN

LYS

GLU

LYS

4

LYS

ILE

PHE

GLN

ILE

PRO

TRP

MET

HIS

ALA

5

ALA

ARG

HIS

GLY

TRP

ASP

VAL

GLU

LYS

ASP

6

ALA

PRO

LEU

PHE

ARG

ASN

TRP

ALA

ILE

HIS

7

THR

GLY

LYS

HIS

GLN

PRO

GLY

ILE

ASP

LYS

8

PRO

ASP

PRO

LYS

THR

TRP

LYS

ALA

ASN

PHE

9

ARG

CYS

ALA

MET

ASN

SER

LEU

PRO

ASP

ILE

10

GLU

VAL

LYS

ASP

ARG

SER

ILE

LYS

11

GLY

ASN

ALA

PHE

ARG

VAL

TYR

ARG

MET

12

LEU

PRO

ALA

Samples:

sample_one: IRF-2 DNA binding domain, [U-13C; U-15N], 0.5 – 1.5 mM

sample_two: IRF-2 DNA binding domain, [U-15N], 0.5 – 1.0 mM

sample_three: IRF-2 DNA binding domain, [2H; HD1, HE3, HZ2, HZ3, HH2]-Trp, 1.0 mM; IRF-2 DNA binding domain, [2H; HD1, HD2, HE1, HE2, HZ]-Phe, 1.0 mM

sample_conditions_one: pH: 5.6; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
HNCA	sample_one	not available	not available
HN(CO)CA	sample_one	not available	not available
HNCO	sample_one	not available	not available
HNCACB	sample_one	not available	not available
CBCA(CO)NH	sample_one	not available	not available
1H-15N NOESY	sample_one	not available	not available
1H-13C NOESY	sample_one	not available	not available
HCCH-TOCSY	sample_one	not available	not available
HCCH-COSY	sample_one	not available	not available
2D NOESY	sample_one	not available	not available
2D TOCSY	sample_one	not available	not available
(HB)CB(CGCD)HD	sample_one	not available	not available

Software:

NMRPipe -

PIPP -

NMR spectrometers:

Bruker AMX 500 MHz
Bruker DMX 500 MHz
Bruker JEOL 600 MHz

PDB	1IRF 1IRG 2IRF
DBJ	BAE32998 BAE34593 BAG35793 BAG73302
EMBL	CAA34073 CAD35751 CAG33358 CAH91897
GB	AAA39333 AAF34781 AAH06577 AAH15803 AAI10667
REF	NP_001009740 NP_001040551 NP_001127478 NP_001129265 NP_001192722
SP	P14316 P23906 Q8R4E0
TPG	DAA14554
AlphaFold	P14316 P23906 Q8R4E0