BMRB Entry 4127

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4127

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Sequence-specific 1H, 13C, and 15N Resonance Assignment of the Origin DNA-binding Domain of SV40 T Antigen

Deposition date:

1998-03-20

Original release date:

1999-03-04

Authors:

Luo, Xuelian; Sanford, David; Bullock, Peter; Bachovchin, William

Citation:

Citation: Luo, Xuelian; Sanford, David; Bullock, Peter; Bachovchin, William. "Solution Structure of the Origin DNA-binding Domain of SV40 T-antigen" Nat. Struct. Biol. 3, 1034-1039 (1996).

Assembly members:

Assembly members:
Origin DNA-binding domain of SV40 T-antigen, polymer, 134 residues, 15390 Da.

Natural source:

Natural source: Common Name: simian virus 40 Taxonomy ID: 10633 Superkingdom: not available Kingdom: not available Genus/species: Polyomavirus Simian virus 40

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-1lamdaT

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Origin DNA-binding domain of SV40 T-antigen: GSKVEDPKDFPSELLSFLSH AVFSNRTLACFAIYTTKEKA ALLYKKIMEKYSVTFISRHN SYNHNILFFLTPHRHRVSAI NNYAQKLCTFSFLICKGVNK EYLMYSALTRDPFSVIEESL PGGLKEHDFNPESS

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_one

Data type	Count
13C chemical shifts	475
15N chemical shifts	130
1H chemical shifts	885

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	T-Ag_OBD	1

Entities:

Entity 1, T-Ag_OBD 134 residues - 15390 Da.

1

GLY

SER

LYS

VAL

GLU

ASP

PRO

LYS

ASP

PHE

2

PRO

SER

GLU

LEU

SER

PHE

LEU

SER

HIS

3

ALA

VAL

PHE

SER

ASN

ARG

THR

LEU

ALA

CYS

4

PHE

ALA

ILE

TYR

THR

LYS

GLU

LYS

ALA

5

ALA

LEU

TYR

LYS

ILE

MET

GLU

LYS

6

TYR

SER

VAL

THR

PHE

ILE

SER

ARG

HIS

ASN

7

SER

TYR

ASN

HIS

ASN

ILE

LEU

PHE

LEU

8

THR

PRO

HIS

ARG

HIS

ARG

VAL

SER

ALA

ILE

9

ASN

TYR

ALA

GLN

LYS

LEU

CYS

THR

PHE

10

SER

PHE

LEU

ILE

CYS

LYS

GLY

VAL

ASN

LYS

11

GLU

TYR

LEU

MET

TYR

SER

ALA

LEU

THR

ARG

12

ASP

PRO

PHE

SER

VAL

ILE

GLU

SER

LEU

13

PRO

GLY

LEU

LYS

GLU

HIS

ASP

PHE

ASN

14

PRO

GLU

SER

Samples:

sample_one: Origin DNA-binding domain of SV40 T-antigen, [U-15N;U-13C], 3 mM

sample_conditions_one: pH: 5.5 na; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions

Software:

No software information available

NMR spectrometers:

unknown unknown 0 MHz

PDB	1TBD 1Z1D 2FUF 2IF9 2IPR 2ITJ 2ITL 2NL8 2NTC 2TBD 3QK2 3QN2 4FGN 4GDF 5D9I
EMBL	CAB82893 CBL79143
GB	AAB59924 AAC59346 AAD38846 AAD38993 AAD39001
REF	YP_003708382
SP	P03070
AlphaFold	P03070