BMRB Entry 4111

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4111

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Title:
Sequential Assignment of the Triple Labelled 30 kDa Cell-adhesion Domain from Enteropathogenic E.coli
Deposition date:
1998-02-24
Original release date:
1999-01-21
Authors:
Kelly, Geoff; Prasannan, Sunil; Daniel, Sarah; Frankel, Gad; Dougan, Gordon; Connerton, Iain; Matthews, Stephen
Citation:

Citation: Kelly, Geoff; Prasannan, Sunil; Daniel, Sarah; Frankel, Gad; Dougan, Gordon; Connerton, Iain; Matthews, Stephen. "Sequential Assignment of the Triple Labelled 30.1 kDa Cell-adhesion Domain of Intimin from Enteropathogenic E. coli"  J Biomol. NMR 12, 189-191 (1998).

Assembly members:

Assembly members:
Intimin cell adhesion domain, polymer, 280 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia Escherichia coli   Vector: pET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Intimin cell adhesion domain: ITEIKADKTTAVANGQDAIT YTVKVMKGDKPVSNQEVTFT TTLGKLSNSTEKTDTNGYAK VTLTSTTPGKSLVSARVSDV AVDVKAPEVEFFTTLTIDDG NIEIVGTGVKGKLPTVWLQY GQVNLKASGGNGKYTWRSAN PAIASVDASSGQVTLKEKGT TTISVISSDNQTATYTIATP NSLIVPNMSKRVTYNDAVNT CKNFGGKLPSSQNELENVFK AWGAANKYEYYKSSQTIISW VQQTAQDAKSGVASTYDLVK QNPLNNIKASESNAYATCVK

Data sets:
Data typeCount
13C chemical shifts478
15N chemical shifts247
1H chemical shifts247

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Int-CAD1

Entities:

Entity 1, Int-CAD 280 residues - Formula weight is not available

1   ILETHRGLUILELYSALAASPLYSTHRTHR
2   ALAVALALAASNGLYGLNASPALAILETHR
3   TYRTHRVALLYSVALMETLYSGLYASPLYS
4   PROVALSERASNGLNGLUVALTHRPHETHR
5   THRTHRLEUGLYLYSLEUSERASNSERTHR
6   GLULYSTHRASPTHRASNGLYTYRALALYS
7   VALTHRLEUTHRSERTHRTHRPROGLYLYS
8   SERLEUVALSERALAARGVALSERASPVAL
9   ALAVALASPVALLYSALAPROGLUVALGLU
10   PHEPHETHRTHRLEUTHRILEASPASPGLY
11   ASNILEGLUILEVALGLYTHRGLYVALLYS
12   GLYLYSLEUPROTHRVALTRPLEUGLNTYR
13   GLYGLNVALASNLEULYSALASERGLYGLY
14   ASNGLYLYSTYRTHRTRPARGSERALAASN
15   PROALAILEALASERVALASPALASERSER
16   GLYGLNVALTHRLEULYSGLULYSGLYTHR
17   THRTHRILESERVALILESERSERASPASN
18   GLNTHRALATHRTYRTHRILEALATHRPRO
19   ASNSERLEUILEVALPROASNMETSERLYS
20   ARGVALTHRTYRASNASPALAVALASNTHR
21   CYSLYSASNPHEGLYGLYLYSLEUPROSER
22   SERGLNASNGLULEUGLUASNVALPHELYS
23   ALATRPGLYALAALAASNLYSTYRGLUTYR
24   TYRLYSSERSERGLNTHRILEILESERTRP
25   VALGLNGLNTHRALAGLNASPALALYSSER
26   GLYVALALASERTHRTYRASPLEUVALLYS
27   GLNASNPROLEUASNASNILELYSALASER
28   GLUSERASNALATYRALATHRCYSVALLYS

Samples:

sample_one: Intimin cell adhesion domain, [U-100% 15N; U-100% 13C, U-80% 2H], 1.0 mM; H2O 90%; D2O 10%

sample_conditions_one: pH: 5.0 na; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Bruker DRX-500 500 MHz

Related Database Links:

PDB
EMBL CAG29634 CAG29721 CAG29722 CAG29769 CAG29783
GB AAL83295 ACB11562 ACX48507 ACX48508

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks