BMRB Entry 4064

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4064

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Assignments, Secondary Structure and Dynamics of the Inhibitor-Free Catalytic Fragment of Human Fibroblast Collagenase

Deposition date:

1997-01-28

Original release date:

1998-02-25

Authors:

Moy, Franklin; Pisano, Michael; Chanda, Pranab; Urbano, Charlotte; Killar, Loran; Sung, Mei-Li; Powers, Robert

Citation:

Citation: Moy, Franklin; Pisano, Michael; Chanda, Pranab; Urbano, Charlotte; Killar, Loran; Sung, Mei-Li; Powers, Robert. "Assignments, Secondary Structure and Dynamics of the Inhibitor-Free Catalytic Fragment of Human Fibroblast Collagenase" J. Biomol. NMR 10, 9-19 (1997).

Assembly members:

Assembly members:
human fibroblast collagenase, polymer, 169 residues, 18700 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pNOT-3A

Entity Sequences (FASTA):

Entity Sequences (FASTA):
human fibroblast collagenase: VLTEGNPRWEQTHLTYRIEN YTPDLPRADVDHAIEKAFQL WSNVTPLTFTKVSEGQADIM ISFVRGDHRDNSPFDGPGGN LAHAFQPGPGIGGDAHFDED ERWTNNFREYNLHRVAAHEL GHSLGLSHSTDIGALMYPSY TFSGDVQLAQDDIDGIQAIY GRSQNPVQP

Data sets:

assigned_chemical_shifts
- chemical_shift_assignment

Data type	Count
13C chemical shifts	716
15N chemical shifts	158
1H chemical shifts	1094

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	collagenase	1

Entities:

Entity 1, collagenase 169 residues - 18700 Da.

1

VAL

LEU

THR

GLU

GLY

ASN

PRO

ARG

TRP

GLU

2

GLN

THR

HIS

LEU

THR

TYR

ARG

ILE

GLU

ASN

3

TYR

THR

PRO

ASP

LEU

PRO

ARG

ALA

ASP

VAL

4

ASP

HIS

ALA

ILE

GLU

LYS

ALA

PHE

GLN

LEU

5

TRP

SER

ASN

VAL

THR

PRO

LEU

THR

PHE

THR

6

LYS

VAL

SER

GLU

GLY

GLN

ALA

ASP

ILE

MET

7

ILE

SER

PHE

VAL

ARG

GLY

ASP

HIS

ARG

ASP

8

ASN

SER

PRO

PHE

ASP

GLY

PRO

GLY

ASN

9

LEU

ALA

HIS

ALA

PHE

GLN

PRO

GLY

PRO

GLY

10

ILE

GLY

ASP

ALA

HIS

PHE

ASP

GLU

ASP

11

GLU

ARG

TRP

THR

ASN

PHE

ARG

GLU

TYR

12

ASN

LEU

HIS

ARG

VAL

ALA

HIS

GLU

LEU

13

GLY

HIS

SER

LEU

GLY

LEU

SER

HIS

SER

THR

14

ASP

ILE

GLY

ALA

LEU

MET

TYR

PRO

SER

TYR

15

THR

PHE

SER

GLY

ASP

VAL

GLN

LEU

ALA

GLN

16

ASP

ILE

ASP

GLY

ILE

GLN

ALA

ILE

TYR

17

GLY

ARG

SER

GLN

ASN

PRO

VAL

GLN

PRO

Samples:

sample_one: human fibroblast collagenase, [U-15N], 1 mM; H2O 90%; D2O 10%; NaCl 100 mM; CaCl2 5 mM; ZnCl2 .1 mM; NaN3 2 mM; deuterated-DTT 10 mM; deuterated-Tris_base 10 mM

sample_two: human fibroblast collagenase, [U-13C;U-15N], 1 mM; D2O 100%; NaCl 100 mM; CaCl2 5 mM; ZnCl2 .1 mM; NaN3 2 mM; deuterated-DTT 10 mM; deuterated-Tris_base 10 mM

sample_conditions: pH: 6.5; temperature: 308 K

Experiments:

Name	Sample	Sample state	Sample conditions

Software:

No software information available

NMR spectrometers:

Bruker AMX600 600 MHz

PDB	1AYK 1CGE 1CGF 1CGL 1HFC 1SU3 2AYK 2CLT 2J0T 2TCL 3AYK 3SHI 4AUO 4AYK 966C
DBJ	BAD96754 BAD96755 BAD96756 BAD96776 BAD97001
EMBL	CAA28858 CAA38691
GB	AAA35699 AAB34887 AAB36941 AAH13875 AAP35520
REF	NP_001139410 NP_002412 XP_002822446 XP_003253096 XP_003253097
SP	P03956
AlphaFold	P03956