BMRB Entry 36272
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR36272
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Citation: Xue, Wei; Zhang, Shu-Xian; He, Wen-Tian; Hong, Jun-Ye; Jiang, Lei-Lei; Hu, Hong-Yu. "Domain interactions reveal auto-inhibition of the deubiquitinating enzyme USP19 and its activation by HSP90 in the modulation of huntingtin aggregation." Biochem. J. 477, 4295-4312 (2020).
PubMed: 33094816
Assembly members:
Ubiquitin carboxyl-terminal hydrolase 19, polymer, 114 residues, 13338.205 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Ubiquitin carboxyl-terminal hydrolase 19: VDEPESMVNLAFVKNDSYEK
GPDSVVVHVYVKEICRDTSR
VLFREQDFTLIFQTRDGNFL
RLHPGCGPHTTFRWQVKLRN
LIEPEQCTFCFTASRIDICL
RKRQSQRWGGLEAP
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 378 |
| 15N chemical shifts | 110 |
| 1H chemical shifts | 491 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
Entities:
Entity 1, entity_1 114 residues - 13338.205 Da.
| 1 | VAL | ASP | GLU | PRO | GLU | SER | MET | VAL | ASN | LEU | ||||
| 2 | ALA | PHE | VAL | LYS | ASN | ASP | SER | TYR | GLU | LYS | ||||
| 3 | GLY | PRO | ASP | SER | VAL | VAL | VAL | HIS | VAL | TYR | ||||
| 4 | VAL | LYS | GLU | ILE | CYS | ARG | ASP | THR | SER | ARG | ||||
| 5 | VAL | LEU | PHE | ARG | GLU | GLN | ASP | PHE | THR | LEU | ||||
| 6 | ILE | PHE | GLN | THR | ARG | ASP | GLY | ASN | PHE | LEU | ||||
| 7 | ARG | LEU | HIS | PRO | GLY | CYS | GLY | PRO | HIS | THR | ||||
| 8 | THR | PHE | ARG | TRP | GLN | VAL | LYS | LEU | ARG | ASN | ||||
| 9 | LEU | ILE | GLU | PRO | GLU | GLN | CYS | THR | PHE | CYS | ||||
| 10 | PHE | THR | ALA | SER | ARG | ILE | ASP | ILE | CYS | LEU | ||||
| 11 | ARG | LYS | ARG | GLN | SER | GLN | ARG | TRP | GLY | GLY | ||||
| 12 | LEU | GLU | ALA | PRO |
Samples:
15N_13C_sample: Ubiquitin carboxyl-terminal hydrolase 19, [U-13C; U-15N], mM; sodium azide 0.05 mM; sodium chloride 50 mM; sodium phosphate 20 mM; H2O 90 % v/v; D2O, [U-2H], 10 % v/v
15N_sample: Ubiquitin carboxyl-terminal hydrolase 19, [U-13C; U-15N], mM; sodium azide 0.05 mM; sodium chloride 50 mM; sodium phosphate 20 mM; H2O 90 % v/v; D2O, [U-2H], 10 % v/v
sample_conditions_1: ionic strength: 0.08 M; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | 15N_sample | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | 15N_13C_sample | isotropic | sample_conditions_1 |
| 3D HNCACB | 15N_13C_sample | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | 15N_13C_sample | isotropic | sample_conditions_1 |
| 3D HNHA | 15N_sample | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | 15N_13C_sample | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | 15N_13C_sample | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | 15N_sample | isotropic | sample_conditions_1 |
| 3D HNCO | 15N_13C_sample | isotropic | sample_conditions_1 |
Software:
NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
Sparky, Goddard - data analysis
Sparky, Goddard - chemical shift assignment
CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure calculation
ARIA, Linge, O'Donoghue and Nilges - structure calculation
CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement
ARIA, Linge, O'Donoghue and Nilges - refinement
NMR spectrometers:
- Bruker AVANCE 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks