BMRB Entry 36122
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR36122
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Zheng, Zhen; Zhang, Meilan; Wang, Yongheng; Ma, Rongsheng; Guo, Chenyun; Feng, Liubin; Wu, Jihui; Yao, Hongwei; Lin, Donghai. "Structural basis for the complete resistance of the human prion protein mutant G127V to prion disease." Sci. Rep. 8, 13211-13211 (2018).
PubMed: 30181558
Assembly members:
Major prion protein, polymer, 142 residues, 16285.22 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli-Pichia pastoris shuttle vector pPpARG4
Entity Sequences (FASTA):
Major prion protein: MQGGGTHSQWNKPSKPKTNM
KHMAGAAAAGAVVGGLGVYM
LGSAMSRPIIHFGSDYEDRY
YRENMHRYPNQVYYRPMDEY
SNQNNFVHDCVNITIKQHTV
TTTTKGENFTETDVKMMERV
VEQMCITQYERESQAYYQRG
SS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 532 |
| 15N chemical shifts | 147 |
| 1H chemical shifts | 900 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
Entities:
Entity 1, entity_1 142 residues - 16285.22 Da.
| 1 | MET | GLN | GLY | GLY | GLY | THR | HIS | SER | GLN | TRP | ||||
| 2 | ASN | LYS | PRO | SER | LYS | PRO | LYS | THR | ASN | MET | ||||
| 3 | LYS | HIS | MET | ALA | GLY | ALA | ALA | ALA | ALA | GLY | ||||
| 4 | ALA | VAL | VAL | GLY | GLY | LEU | GLY | VAL | TYR | MET | ||||
| 5 | LEU | GLY | SER | ALA | MET | SER | ARG | PRO | ILE | ILE | ||||
| 6 | HIS | PHE | GLY | SER | ASP | TYR | GLU | ASP | ARG | TYR | ||||
| 7 | TYR | ARG | GLU | ASN | MET | HIS | ARG | TYR | PRO | ASN | ||||
| 8 | GLN | VAL | TYR | TYR | ARG | PRO | MET | ASP | GLU | TYR | ||||
| 9 | SER | ASN | GLN | ASN | ASN | PHE | VAL | HIS | ASP | CYS | ||||
| 10 | VAL | ASN | ILE | THR | ILE | LYS | GLN | HIS | THR | VAL | ||||
| 11 | THR | THR | THR | THR | LYS | GLY | GLU | ASN | PHE | THR | ||||
| 12 | GLU | THR | ASP | VAL | LYS | MET | MET | GLU | ARG | VAL | ||||
| 13 | VAL | GLU | GLN | MET | CYS | ILE | THR | GLN | TYR | GLU | ||||
| 14 | ARG | GLU | SER | GLN | ALA | TYR | TYR | GLN | ARG | GLY | ||||
| 15 | SER | SER |
Samples:
sample_2: HuPrP(G127V), [U-99% 13C; U-99% 15N], 0.5 mM; H2O 90%; D2O, [U-2H], 10%
sample_conditions_1: ionic strength: 20 mM; pH: 4.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | not available | isotropic | sample_conditions_1 |
| 3D HNCACB | not available | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | not available | isotropic | sample_conditions_1 |
| 3D HNCA | not available | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | not available | isotropic | sample_conditions_1 |
| 3D HNCO | not available | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | not available | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | not available | isotropic | sample_conditions_1 |
| 3D C(CO)NH | not available | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | not available | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | not available | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aliphatic | not available | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | not available | isotropic | sample_conditions_1 |
Software:
CARA, Keller and Wuthrich - chemical shift assignment
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure calculation
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Bruker AvanceIII 850 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks