BMRB Entry 34963

Name: ZT-KP6-1: AN EFFECTOR FROM ZYMOSEPTORIA TRITICI
Published: 2024-11-05

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34963

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

ZT-KP6-1: AN EFFECTOR FROM ZYMOSEPTORIA TRITICI

Deposition date:

2024-09-26

Original release date:

2024-11-05

Authors:

Barthe, P.; de Guillen, K.

Citation:

Citation: de Guillen, K.; Mammri, L.; Gracy, J.; Padilla, A.; Barthe, P.; Hoh, F.; Lahfa, M.; Rouffet, J.; Petit-Houdenot, Y.; Kroj, T.; Lebrun, M.. "Structural studies identify killer proteins 4 and 6 from the fungal wheat pathogen Zymoseptoria tritici toxic to fungi and related to structural effector families" .

Assembly members:

Assembly members:
entity_1, polymer, 80 residues, 9081.172 Da.

Natural source:

Natural source: Common Name: Zymoseptoria tritici ST99CH_1E4 Taxonomy ID: 1276532 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Zymoseptoria tritici

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GHMAVVYAARCKFGNPLVQN NRITRAVCDLTNEHTTKDGS WHYVEVDNECKYLAGDNPRD QPGWAVFVKYCTYYKGVPDA

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
15N chemical shifts	87
1H chemical shifts	540

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	unit_1	1

Entities:

Entity 1, unit_1 80 residues - 9081.172 Da.

1	GLY	HIS	MET	ALA	VAL	VAL	TYR	ALA	ALA	ARG
2	CYS	LYS	PHE	GLY	ASN	PRO	LEU	VAL	GLN	ASN
3	ASN	ARG	ILE	THR	ARG	ALA	VAL	CYS	ASP	LEU
4	THR	ASN	GLU	HIS	THR	THR	LYS	ASP	GLY	SER
5	TRP	HIS	TYR	VAL	GLU	VAL	ASP	ASN	GLU	CYS
6	LYS	TYR	LEU	ALA	GLY	ASP	ASN	PRO	ARG	ASP
7	GLN	PRO	GLY	TRP	ALA	VAL	PHE	VAL	LYS	TYR
8	CYS	THR	TYR	TYR	LYS	GLY	VAL	PRO	ASP	ALA

Samples:

sample_1: Zt-KP6-1, [U-100% 15N], 0.7 mM; ammonium citrate 20 mM; sodium chloride 150 mM; DTT 1 mM

sample_2: Zt-KP6-1, [U-100% 15N], 0.7 mM; ammonium citrate 20 mM; sodium chloride 150 mM; DTT 1 mM

sample_conditions_1: ionic strength: 150 mM; pH: 5.4; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_2	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_2	isotropic	sample_conditions_1
2D DQF-COSY	sample_2	isotropic	sample_conditions_1

Software:

CINDY v2.1, Padilla, A. - chemical shift assignment, peak picking

CYANA v3.98.15, Guntert, Mumenthaler and Wuthrich - structure calculation

CNS v1.2, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

NMR spectrometers:

Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

1	GLY	HIS	MET	ALA	VAL	VAL	TYR	ALA	ALA	ARG
2	CYS	LYS	PHE	GLY	ASN	PRO	LEU	VAL	GLN	ASN
3	ASN	ARG	ILE	THR	ARG	ALA	VAL	CYS	ASP	LEU
4	THR	ASN	GLU	HIS	THR	THR	LYS	ASP	GLY	SER
5	TRP	HIS	TYR	VAL	GLU	VAL	ASP	ASN	GLU	CYS
6	LYS	TYR	LEU	ALA	GLY	ASP	ASN	PRO	ARG	ASP
7	GLN	PRO	GLY	TRP	ALA	VAL	PHE	VAL	LYS	TYR
8	CYS	THR	TYR	TYR	LYS	GLY	VAL	PRO	ASP	ALA

1	GLY	HIS	MET	ALA	VAL	VAL	TYR	ALA	ALA	ARG
2	CYS	LYS	PHE	GLY	ASN	PRO	LEU	VAL	GLN	ASN
3	ASN	ARG	ILE	THR	ARG	ALA	VAL	CYS	ASP	LEU
4	THR	ASN	GLU	HIS	THR	THR	LYS	ASP	GLY	SER
5	TRP	HIS	TYR	VAL	GLU	VAL	ASP	ASN	GLU	CYS
6	LYS	TYR	LEU	ALA	GLY	ASP	ASN	PRO	ARG	ASP
7	GLN	PRO	GLY	TRP	ALA	VAL	PHE	VAL	LYS	TYR
8	CYS	THR	TYR	TYR	LYS	GLY	VAL	PRO	ASP	ALA

1	GLY	HIS	MET	ALA	VAL	VAL	TYR	ALA	ALA	ARG
2	CYS	LYS	PHE	GLY	ASN	PRO	LEU	VAL	GLN	ASN
3	ASN	ARG	ILE	THR	ARG	ALA	VAL	CYS	ASP	LEU
4	THR	ASN	GLU	HIS	THR	THR	LYS	ASP	GLY	SER
5	TRP	HIS	TYR	VAL	GLU	VAL	ASP	ASN	GLU	CYS
6	LYS	TYR	LEU	ALA	GLY	ASP	ASN	PRO	ARG	ASP
7	GLN	PRO	GLY	TRP	ALA	VAL	PHE	VAL	LYS	TYR
8	CYS	THR	TYR	TYR	LYS	GLY	VAL	PRO	ASP	ALA