BMRB Entry 34946
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34946
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NMR-STAR v3 text file.
XML gzip file.
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All files associated with the entry
Citation: Weininger, U.; Golbik, R.; Thondorf, I.; Behrens, S.. "RNA binding domain of Turnip crinkle virus p38, p38R" .
Assembly members:
entity_1, polymer, 45 residues, 5187.014 Da.
Natural source: Common Name: Turnip crinkle virus Taxonomy ID: 11988 Superkingdom: Viruses Kingdom: not available Genus/species: Betacarmovirus brassicae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity_1: MENDPRVRKFASDGAQWAIK
WQKKGWSTLTSRQKQTARAA
MGIKL
- assigned_chemical_shifts
- spectral_peak_list
| Data type | Count |
| 13C chemical shifts | 161 |
| 15N chemical shifts | 51 |
| 1H chemical shifts | 330 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | unit_1 | 1 |
Entities:
Entity 1, unit_1 45 residues - 5187.014 Da.
| 1 | MET | GLU | ASN | ASP | PRO | ARG | VAL | ARG | LYS | PHE | ||||
| 2 | ALA | SER | ASP | GLY | ALA | GLN | TRP | ALA | ILE | LYS | ||||
| 3 | TRP | GLN | LYS | LYS | GLY | TRP | SER | THR | LEU | THR | ||||
| 4 | SER | ARG | GLN | LYS | GLN | THR | ALA | ARG | ALA | ALA | ||||
| 5 | MET | GLY | ILE | LYS | LEU |
Samples:
sample_1: entity_1, [U-100% 13C; U-100% 15N], 1.0 mM; sodium phosphate 50 mM
sample_conditions_1: ionic strength: 50 mM; pH: 7.0; pressure: 1 bar; temperature: 283 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aliphatic | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aromatic | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aliphatic | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aromatic | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRViewJ, Johnson, One Moon Scientific - chemical shift assignment, peak picking
CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure calculation
ARIA, Linge, O'Donoghue and Nilges - structure calculation
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Bruker AVANCE III 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks