BMRB Entry 34939

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34939

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Title:
N2 domain of g3p from phage fd
Deposition date:
2024-07-23
Original release date:
2025-05-29
Authors:
Weininger, U.; Jakob, R.
Citation:

Citation: Weininger, U.; Jakob, R.. "N2 domain of g3p from phage fd"  .

Assembly members:

Assembly members:
entity_1, polymer, 110 residues, 12730.849 Da.

Natural source:

Natural source:   Common Name: Enterobacteria phage fd   Taxonomy ID: 10864   Superkingdom: Viruses   Kingdom: not available   Genus/species: Inovirus Enterobacteria phage fd

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: YINPLDGTYPPGTEQNPANP NPSLEESHPLNTFMFQNNRF RNRQGALTVYTGTVTQGTDP VKTYYQYTPVSSKAMYDAYW NGKFRDCAFHSGFNEDLFVC EYQGHHHHHH

Data sets:
Data typeCount
13C chemical shifts270
15N chemical shifts112
1H chemical shifts731

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 110 residues - 12730.849 Da.

1   TYRILEASNPROLEUASPGLYTHRTYRPRO
2   PROGLYTHRGLUGLNASNPROALAASNPRO
3   ASNPROSERLEUGLUGLUSERHISPROLEU
4   ASNTHRPHEMETPHEGLNASNASNARGPHE
5   ARGASNARGGLNGLYALALEUTHRVALTYR
6   THRGLYTHRVALTHRGLNGLYTHRASPPRO
7   VALLYSTHRTYRTYRGLNTYRTHRPROVAL
8   SERSERLYSALAMETTYRASPALATYRTRP
9   ASNGLYLYSPHEARGASPCYSALAPHEHIS
10   SERGLYPHEASNGLUASPLEUPHEVALCYS
11   GLUTYRGLNGLYHISHISHISHISHISHIS

Samples:

sample_1: N2 domain of g3p from phage fd, [U-100% 13C; U-100% 15N], 1.0 mM; potassium phosphate 100 mM

sample_conditions_1: ionic strength: 100 mM; pH: 7.0; pressure: 1 bar; temperature: 288.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure calculation

NMR spectrometers:

  • Bruker AVANCE II 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks