BMRB Entry 26995

Name: A consensus homeodomain
Published: 2017-03-31

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26995

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

A consensus homeodomain

Deposition date:

2017-01-15

Original release date:

2017-03-31

Authors:

Sternke, Matt; Barrick, Doug; Tripp, Katherine; Majumdar, Ananya

Citation:

Citation: Tripp, Katherine; Sternke, Matt; Majumdar, Ananya; Barrick, Doug; Tripp, Katherine; Sternke, Matt; Majumdar, Ananya; Barrick, Doug. "Assigned Chemical Shifts for a consensus homeodomain" .

Assembly members:

Assembly members:
Consensus_homeomdomain, polymer, 66 residues, Formula weight is not available

Natural source:

Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET24

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Consensus_homeomdomain: MGSRRKRTTFTKEQLEELEE LFEKNRYPSAEEREELAKKL GLTERQVKVWFQNRRAKEKK HHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	249
15N chemical shifts	55
1H chemical shifts	375

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Consensus homeomdomain, subunit 1	1
2	Consensus homeomdomain, subunit 2	1

Entities:

Entity 1, Consensus homeomdomain, subunit 1 66 residues - Formula weight is not available

1

MET

GLY

SER

ARG

LYS

ARG

THR

PHE

2

THR

LYS

GLU

GLN

LEU

GLU

LEU

GLU

3

LEU

PHE

GLU

LYS

ASN

ARG

TYR

PRO

SER

ALA

4

GLU

ARG

GLU

LEU

ALA

LYS

LEU

5

GLY

LEU

THR

GLU

ARG

GLN

VAL

LYS

VAL

TRP

6

PHE

GLN

ASN

ARG

ALA

LYS

GLU

LYS

7

HIS

Samples:

sample_1: Consensus homeomdomain, [U-100% 13C; U-100% 15N], 400 uM; potassium phosphate 25 mM; potassium chloride 150 mM

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D (H)CC(CO)NH-TOCSY	sample_1	isotropic	sample_conditions_1
3D H(CCCO)NH-TOCSY	sample_1	isotropic	sample_conditions_1
3D HN(CA)N	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment, peak picking

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks