BMRB Entry 26346

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for FOXO3a (141-284)
Published: 2025-09-15

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26346

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for FOXO3a (141-284)

Deposition date:

2024-07-12

Original release date:

2025-09-15

Authors:

Enomoto, Shota; Nakatsuka, Shoichi; Yokogawa, Mariko; Osawa, Masanori

Citation:

Citation: Enomoto, Shota; Nakatsuka, Shoichi; Kuwayama, Tomoya; Kawatsu, Kosaku; Yokogawa, Mariko; Osawa, Masanori. "NMR (1)H, (13)C, (15)N backbone resonance assignments of 14-3-3z binding region of human FOXO3a (residues 1-284)." Biomol. NMR Assign. 18, 275-283 (2024).
PubMed: 39259470

Assembly members:

Assembly members:
FOXO3a (141-284), polymer, 149 residues, 16404 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX6P-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FOXO3a (141-284): GPLGSAGGSGQPRKCSSRRN AWGNLSYADLITRAIESSPD KRLTLSQIYEWMVRCVPYFK DKGDSNSSAGWKNSIRHNLS LHSRFMRVQNEGTGKSSWWI INPDGGKSGKAPRRRAVSMD NSNKYTKSRGRAAKKKAALQ TAPESADDS

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	444
15N chemical shifts	132
1H chemical shifts	132

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	subunit 1	1

Entities:

Entity 1, subunit 1 149 residues - 16404 Da.

1

GLY

PRO

LEU

GLY

SER

ALA

GLY

SER

GLY

2

GLN

PRO

ARG

LYS

CYS

SER

ARG

ASN

3

ALA

TRP

GLY

ASN

LEU

SER

TYR

ALA

ASP

LEU

4

ILE

THR

ARG

ALA

ILE

GLU

SER

PRO

ASP

5

LYS

ARG

LEU

THR

LEU

SER

GLN

ILE

TYR

GLU

6

TRP

MET

VAL

ARG

CYS

VAL

PRO

TYR

PHE

LYS

7

ASP

LYS

GLY

ASP

SER

ASN

SER

ALA

GLY

8

TRP

LYS

ASN

SER

ILE

ARG

HIS

ASN

LEU

SER

9

LEU

HIS

SER

ARG

PHE

MET

ARG

VAL

GLN

ASN

10

GLU

GLY

THR

GLY

LYS

SER

TRP

ILE

11

ILE

ASN

PRO

ASP

GLY

LYS

SER

GLY

LYS

12

ALA

PRO

ARG

ALA

VAL

SER

MET

ASP

13

ASN

SER

ASN

LYS

TYR

THR

LYS

SER

ARG

GLY

14

ARG

ALA

LYS

ALA

LEU

GLN

15

THR

ALA

PRO

GLU

SER

ALA

ASP

SER

Samples:

sample_1: FOXO3a (141-284), [U-13C; U-15N], 567 uM; DTT 1 mM; sodium chloride 100 mM; sodium phosphate 20 mM; D2O, [U-2H], 10%; H2O 90%

sample_conditions_1: pH: 6.4; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D CC(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

SPARKY v3.190 - chemical shift assignment

TOPSPIN v4.2.0 - processing

NMR spectrometers:

Bruker AVANCE NEO 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks