BMRB Entry 19600

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19600
MolProbity Validation Chart

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Title:
CEH37 Homeodomain
Deposition date:
2013-11-04
Original release date:
2014-02-11
Authors:
Moon, Sunjin; Lee, Weontae
Citation:

Citation: Moon, Sunjin; Lee, Yong Woo; Kim, Woo Taek; Lee, Weontae. "Solution structure of CEH-37 homeodomain of the nematode Caenorhabditis elegans."  Biochem. Biophys. Res. Commun. 443, 370-375 (2014).
PubMed: 24361878

Assembly members:

Assembly members:
CEH-37_Homeodomain, polymer, 68 residues, 8511.745 Da.

Natural source:

Natural source:   Common Name: nematode   Taxonomy ID: 6239   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Caenorhabditis elegans

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET32a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CEH-37_Homeodomain: GPRKNRRERTTYSRQQLEIL ETLFNETQYPDVFARERVAD QIRLQESRIQVWFKNRRAKY RLQEKQKP

Data sets:
Data typeCount
13C chemical shifts199
15N chemical shifts64
1H chemical shifts431

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1CEH37 Homeodomain1

Entities:

Entity 1, CEH37 Homeodomain 68 residues - 8511.745 Da.

1   GLYPROARGLYSASNARGARGGLUARGTHR
2   THRTYRSERARGGLNGLNLEUGLUILELEU
3   GLUTHRLEUPHEASNGLUTHRGLNTYRPRO
4   ASPVALPHEALAARGGLUARGVALALAASP
5   GLNILEARGLEUGLNGLUSERARGILEGLN
6   VALTRPPHELYSASNARGARGALALYSTYR
7   ARGLEUGLNGLULYSGLNLYSPRO

Samples:

sample_1: CEH-37 Homeodomain, [U-99% 13C; U-99% 15N], 1 mM; H2O 90%; D2O, [U-100% 2H], 10%

conditions_1: pH: 7.5; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicconditions_1
3D CBCA(CO)NHsample_1isotropicconditions_1
3D HNCACBsample_1isotropicconditions_1
3D HNCOsample_1isotropicconditions_1
3D HNCAsample_1isotropicconditions_1
3D HBHA(CO)NHsample_1isotropicconditions_1
3D 1H-15N NOESYsample_1isotropicconditions_1
3D HCCH-TOCSYsample_1isotropicconditions_1
3D 1H-13C NOESYsample_1isotropicconditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker DRX 800 MHz

Related Database Links:

PDB
EMBL CAB02825 CDX47432 CDX47433
GB ACV72913 ACV72914 ACV72915 ACV72916 ACV72917
REF NP_001294799 NP_001294800 NP_510366 XP_003102040
SP Q93356
AlphaFold Q93356

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks