BMRB Entry 18898

Name: Backbone and side-chain assignments of a tethered complex between LMO4 and DEAF-1
Published: 2013-02-21

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18898

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone and side-chain assignments of a tethered complex between LMO4 and DEAF-1

Deposition date:

2012-12-13

Original release date:

2013-02-21

Authors:

Joseph, Soumya; Kwan, Ann; Mackay, Joel; Cubeddu, Liza; Matthews, Jacqueline

Citation:

Citation: Joseph, Soumya; Kwan, Ann; Mackay, Joel; Cubeddu, Liza; Matthews, Jacqueline. "Backbone and side-chain assignments of a tethered complex between LMO4 and DEAF-1." Biomol. NMR Assignments ., .-. (2013).
PubMed: 23417771

Assembly members:

Assembly members:
LMO4-DEAF-1, polymer, 96 residues, Formula weight is not available
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-2T

Entity Sequences (FASTA):

Entity Sequences (FASTA):
LMO4-DEAF-1: GSYIRLFGNSGACSACGQSI PASELVMRAQGNVYHLKCFT CSTCRNRLVPGDRFHYINGS LFCEHDRPTALINGGSGGSG SIAPFPEAALPTSHPK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	354
15N chemical shifts	103
1H chemical shifts	608

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	LMO4-DEAF-1 tethered complex	1
2	Zn2+, 1	2
3	Zn2+, 2	2

Entities:

Entity 1, LMO4-DEAF-1 tethered complex 96 residues - Formula weight is not available

G1 and S2 are non-native residues that remain from thrombin cleavage of the affinity tag. Residues Y3-N73 correspond to murine LMO4 residues 77-147. Residues G74-S81 correspond to a synthetic G/S linker peptide. Residues I82-K96 corresond to murine DEAF1 residues 404-418.

1

GLY

SER

TYR

ILE

ARG

LEU

PHE

GLY

ASN

SER

2

GLY

ALA

CYS

SER

ALA

CYS

GLY

GLN

SER

ILE

3

PRO

ALA

SER

GLU

LEU

VAL

MET

ARG

ALA

GLN

4

GLY

ASN

VAL

TYR

HIS

LEU

LYS

CYS

PHE

THR

5

CYS

SER

THR

CYS

ARG

ASN

ARG

LEU

VAL

PRO

6

GLY

ASP

ARG

PHE

HIS

TYR

ILE

ASN

GLY

SER

7

LEU

PHE

CYS

GLU

HIS

ASP

ARG

PRO

THR

ALA

8

LEU

ILE

ASN

GLY

SER

GLY

SER

GLY

9

SER

ILE

ALA

PRO

PHE

PRO

GLU

ALA

LEU

10

PRO

THR

SER

HIS

PRO

LYS

Entity 2, Zn2+, 1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_LMO4-DEAF-1: LMO4-DEAF-1, [U-13C; U-15N], 0.5 ± 0.2 mM; LMO4-DEAF-1, [U-15N], 0.5 ± 0.2 mM; LMO4-DEAF-1 0.5 ± 0.2 mM; sodium acetate 20 ± 1 mM; sodium chloride 35 ± 1 mM; TCEP 1 ± 0.5 mM; H2O 55 ± 1 M; D2O 5 ± 1 M

condition_LMO4-DEAF-1: ionic strength: 0.1 M; pH: 5.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_LMO4-DEAF-1	isotropic	condition_LMO4-DEAF-1
2D 1H-13C HSQC	sample_LMO4-DEAF-1	isotropic	condition_LMO4-DEAF-1
2D 1H-1H TOCSY	sample_LMO4-DEAF-1	isotropic	condition_LMO4-DEAF-1
2D DQF-COSY	sample_LMO4-DEAF-1	isotropic	condition_LMO4-DEAF-1
2D 1H-1H NOESY	sample_LMO4-DEAF-1	isotropic	condition_LMO4-DEAF-1
3D CBCA(CO)NH	sample_LMO4-DEAF-1	isotropic	condition_LMO4-DEAF-1
3D HNCO	sample_LMO4-DEAF-1	isotropic	condition_LMO4-DEAF-1
3D HNCACB	sample_LMO4-DEAF-1	isotropic	condition_LMO4-DEAF-1
3D H(CCO)NH	sample_LMO4-DEAF-1	isotropic	condition_LMO4-DEAF-1
3D HCCH-TOCSY	sample_LMO4-DEAF-1	isotropic	condition_LMO4-DEAF-1
3D 1H-15N NOESY	sample_LMO4-DEAF-1	isotropic	condition_LMO4-DEAF-1
3D 1H-13C NOESY aliphatic	sample_LMO4-DEAF-1	isotropic	condition_LMO4-DEAF-1

Software:

SPARKY, Goddard - chemical shift assignment, data analysis

NMR spectrometers:

Bruker Avance 600 MHz
Bruker Avance 800 MHz

BMRB	19415
PDB	2MBV
DBJ	BAC37938 BAE23609 BAE35210 BAF85267 BAF93844
EMBL	CAF90424 CAJ82708
GB	AAB51073 AAC62958 AAC83789 AAC98510 AAH03488
REF	NP_001004922 NP_001009708 NP_001029923 NP_001087890 NP_001106156
SP	P61968 P61969 Q3SWZ8 Q6DJ06
TPG	DAA31414
AlphaFold	P61968 P61969 Q3SWZ8 Q6DJ06