BMRB Entry 16803

Name: 1H, 13C and 15N backbone and side chain resonance assignment of the human RANTES-E66S mutant in a dimeric form
Published: 2010-04-28

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16803

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

1H, 13C and 15N backbone and side chain resonance assignment of the human RANTES-E66S mutant in a dimeric form

Deposition date:

2010-03-30

Original release date:

2010-04-28

Authors:

Bieri, Andrej; Grzesiek, Stephan

Citation:

Citation: Bieri, Andrej; Grzesiek, Stephan. "1H, 13C and 15N backbone and side chain resonance assignment of the human RANTES-E66S mutant in a dimeric form" .

Assembly members:

Assembly members:
RANTES-E66S, polymer, 68 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pEGV2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RANTES-E66S: SPYSSDTTPCCFAYIARPLP RAHIKEYFYTSGKCSNPAVV FVTRKNRQVCANPEKKWVRE YINSLSMS

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	310
15N chemical shifts	67
1H chemical shifts	473

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	polymer	1

Entities:

Entity 1, polymer 68 residues - Formula weight is not available

1

SER

PRO

TYR

SER

ASP

THR

PRO

CYS

2

CYS

PHE

ALA

TYR

ILE

ALA

ARG

PRO

LEU

PRO

3

ARG

ALA

HIS

ILE

LYS

GLU

TYR

PHE

TYR

THR

4

SER

GLY

LYS

CYS

SER

ASN

PRO

ALA

VAL

5

PHE

VAL

THR

ARG

LYS

ASN

ARG

GLN

VAL

CYS

6

ALA

ASN

PRO

GLU

LYS

TRP

VAL

ARG

GLU

7

TYR

ILE

ASN

SER

LEU

SER

MET

SER

Samples:

sample_1: D2O 5%; H2O 95%; potassium phosphate 50 mM; sodium azide 0.02%; Rantes-E66S, [U-100% 13C; U-100% 15N], 2 mM

sample_2: D2O 100%; potassium phosphate 50 mM; Rantes-E66S, [U-100% 15N], 0.5 mM

sample_conditions_1: pH: 3.8; pressure: 1 atm; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Bruker DRX 600 MHz
Bruker DRX 800 MHz

BMRB	17453
PDB	1B3A 1EQT 1HRJ 1RTN 1RTO 1U4L 1U4M 1U4P 2L9H
DBJ	BAG35145 BAG73001
GB	AAA36725 AAC03541 AAC63331 AAF73070 AAH08600
REF	NP_002976 XP_002827318 XP_003281422 XP_003818023 XP_008009272
SP	P13501
AlphaFold	P13501