BMRB Entry 16691

Name: Solution NMR Structure of Probable 30S Ribosomal Protein PSRP-3 (Ycf65-like protein) from Synechocystis sp. (strain PCC 6803), Northeast Structural Genomics Consortium Target Target SgR46
Published: 2010-02-25

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PDB ID: 2kt9
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16691
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution NMR Structure of Probable 30S Ribosomal Protein PSRP-3 (Ycf65-like protein) from Synechocystis sp. (strain PCC 6803), Northeast Structural Genomics Consortium Target Target SgR46

Deposition date:

2010-01-22

Original release date:

2010-02-25

Authors:

Liu, G.; Janjua, J.; Xiao, R.; Buchwald, W.; Ciccosanti, C.; Belote, R.; Everett, J.; Nair, R.; Acton, T; Rost, B.; Montelione, G.

Citation:

Citation: Liu, G.; Janjua, J.; Xiao, R.; Buchwald, W.; Ciccosanti, C.; Belote, R.; Everett, J.; Nair, R.; Acton, T; Rost, B.; Montelione, G.. "Solution NMR Structure of Probable 30S ribosomal protein PSRP-3 (Ycf65-like protein) from Synechocystis sp. (PCC 6803), Northeast Structural Genomics Consortium Target Target SgR46" To be published ., .-..

Assembly members:

Assembly members:
SgR46, polymer, 116 residues, 13318.086 Da.

Natural source:

Natural source: Common Name: Synechocystis sp. PCC 6803 Taxonomy ID: 1148 Superkingdom: Bacteria Kingdom: not available Genus/species: Synechocystis Synechocystis sp. PCC 6803

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: BL21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SgR46: MAASTVHTSFILKVLWLDQN VAIAVDQIVGKGTSPLTSYF FWPRADAWQQLKDELEAKHW IAEADRINVLNQATEVINFW QDLKNQNKQISMAEAQGKFP EVVFSGSNLEHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	373
15N chemical shifts	117
1H chemical shifts	783

Time Domain Data

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SgR46	1

Entities:

Entity 1, SgR46 116 residues - 13318.086 Da.

1

MET

ALA

SER

THR

VAL

HIS

THR

SER

PHE

2

ILE

LEU

LYS

VAL

LEU

TRP

LEU

ASP

GLN

ASN

3

VAL

ALA

ILE

ALA

VAL

ASP

GLN

ILE

VAL

GLY

4

LYS

GLY

THR

SER

PRO

LEU

THR

SER

TYR

PHE

5

PHE

TRP

PRO

ARG

ALA

ASP

ALA

TRP

GLN

6

LEU

LYS

ASP

GLU

LEU

GLU

ALA

LYS

HIS

TRP

7

ILE

ALA

GLU

ALA

ASP

ARG

ILE

ASN

VAL

LEU

8

ASN

GLN

ALA

THR

GLU

VAL

ILE

ASN

PHE

TRP

9

GLN

ASP

LEU

LYS

ASN

GLN

ASN

LYS

GLN

ILE

10

SER

MET

ALA

GLU

ALA

GLN

GLY

LYS

PHE

PRO

11

GLU

VAL

PHE

SER

GLY

SER

ASN

LEU

GLU

12

HIS

Samples:

sample: SgR46, [U-100% 13C; U-100% 15N], 1.1 mM; H2O 95%; D2O 5%

sample_1: SgR46, [U-10% 13C; U-100% 15N], 0.8 mM; H2O 95%; D2O 5%

sample_2: SgR46, [U-100% 13C; U-100% 15N], 0.3 mM; H2O 95%; D2O 5%

sample_conditions_1: pH: 7.5; pressure: 1.0 atm; temperature: 293 K

sample_conditions_2: pH: 4.5; pressure: 1.0 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic 927	sample	isotropic	sample_conditions_1
3D HNCO	sample_2	isotropic	sample_conditions_2
3D CBCA(CO)NH 816	sample_2	isotropic	sample_conditions_2
3D HNCACB	sample_2	isotropic	sample_conditions_2
3D 1H-13C arom NOESY	sample	isotropic	sample_conditions_1
3D simutaneous 13C-aromatic,13C-aliphatic,15N edited 1H-1H NOESY	sample	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_2
3D HCCH-TOCSY 829	sample	isotropic	sample_conditions_1
3D HNCA	sample	isotropic	sample_conditions_1
3D CBCA(CO)NH 819	sample_2	isotropic	sample_conditions_2
3D C(CO)NH	sample_2	isotropic	sample_conditions_2
3D HNCOCA	sample_2	isotropic	sample_conditions_2
3D 1H-15N NOESY 939	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY 940	sample_1	isotropic	sample_conditions_1
1D T1	sample_1	isotropic	sample_conditions_1
1D T2	sample_1	isotropic	sample_conditions_1
3D 1H-13C arom TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-13C arom HSQC	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY 830	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic 928	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinemen,structure solution,geometry optimization

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - refinement,geometry optimization,structure solution

AutoStruct v2.1, Huang, Tejero, Powers and Montelione - data analysis,refinement

AutoAssign v2.1, Zimmerman, Moseley, Kulikowski and Montelione - data analysis,chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

XEASY, Bartels et al. - data analysis,peak picking,chemical shift assignment

TOPSPIN, Bruker Biospin - collection

VNMRJ, Varian - collection

NMR spectrometers:

Bruker Avance 800 MHz
Varian INOVA 600 MHz

PDB	2KT9
DBJ	BAA10474 BAK51259 BAL30257 BAL33426 BAL36595
GB	AGF52766 AIE74500 ALJ68680
REF	WP_010873701 WP_038531511
SP	Q55385
AlphaFold	Q55385