BMRB Entry 16402

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16402

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Title:
Solution structure of SCA7 zinc finger domain from human Ataxin-7-L3 protein
Deposition date:
2009-07-10
Original release date:
2010-10-18
Authors:
Wang, Ying-Hui; Atkinson, Andrew; Bonnet, Jacques; Romier, Christophe; Kieffer, Bruno
Citation:

Citation: Bonnet, Jacques; Wang, Ying-Hui; Spedale, Gianpiero; Atkinson, R. Andrew; Romier, Christophe; Hamiche, Ali; Pijnappel, W. W M Pim; Timmers, H. Th Marc; Tora, Laszlo; Devys, Didier; Kieffer, Bruno. "The structural plasticity of SCA7 domains defines their differential nucleosome-binding properties."  EMBO Rep. 11, 612-618 (2010).
PubMed: 20634802

Assembly members:

Assembly members:
SCA7, polymer, 82 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-4T1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SCA7: HMGPEELRSLLTTQCGVISE HTKKMCTRSLRCPQHTDEQR RTVRIYFLGPSAVLPEVESS LDNDSFDMTDSQALISRLQW DG

Data sets:
Data typeCount
13C chemical shifts328
15N chemical shifts91
1H chemical shifts560

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SCA71
2ZINC ION2

Entities:

Entity 1, SCA7 82 residues - Formula weight is not available

1   HISMETGLYPROGLUGLULEUARGSERLEU
2   LEUTHRTHRGLNCYSGLYVALILESERGLU
3   HISTHRLYSLYSMETCYSTHRARGSERLEU
4   ARGCYSPROGLNHISTHRASPGLUGLNARG
5   ARGTHRVALARGILETYRPHELEUGLYPRO
6   SERALAVALLEUPROGLUVALGLUSERSER
7   LEUASPASNASPSERPHEASPMETTHRASP
8   SERGLNALALEUILESERARGLEUGLNTRP
9   ASPGLY

Entity 2, ZINC ION - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: sodium phosphate 50 mM; sodium chloride 200 mM; DTT 2 mM; entity_1, [U-99% 13C; U-99% 15N], 0.3 mM; H2O 90%; D2O 10%

sample_2: sodium phosphate 50 mM; sodium chloride 200 mM; DTT 2 mM; entity_1 0.6 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.3 M; pH: 7.1; pressure: 1 atm; temperature: 295 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker Avance 950 MHz

Related Database Links:

PDB
DBJ BAB71070 BAC41186 BAG59283
EMBL CAB99093
GB AAH37418 AAI13596 AAI26114 ADR82942 AIC51952
REF NP_001092303 NP_001092306 NP_001092307 NP_001179537 NP_064603
SP A2AWT3 Q14CW9
TPG DAA18386
AlphaFold A2AWT3 Q14CW9

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks