BMRB Entry 15548

Name: Backbone assignment of stefin B monomer
Published: 2007-12-11

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15548

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone assignment of stefin B monomer

Deposition date:

2007-11-08

Original release date:

2007-12-11

Authors:

Morgan, Gareth; Hounslow, Andrea; Waltho, Jonathan

Citation:

Citation: Morgan, Gareth; Giannini, Silva; Hounslow, Andrea; Craven, C; Zerovnik, Eva; Turk, Vito; Waltho, Jonathan; Staniforth, Rosemary. "Exclusion of the native alpha-helix from the amyloid fibrils of a mixed alpha/beta protein" J. Mol. Biol. 375, 487-498 (2007).
PubMed: 18021806

Assembly members:

Assembly members:
stefin_B, polymer, 98 residues, 11124 Da.

Natural source:

Natural source: Common Name: not available Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
stefin_B: MMSGAPSATQPATAETQHIA DQVRSQLEEKENKKFPVFKA VSFKSQVVAGTNYFIKVHVG DEDFVHLRVFQSLPHENKPL TLSNYQTNKAKHDELTYF

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	283
15N chemical shifts	91
1H chemical shifts	91

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	stefin B	1

Entities:

Entity 1, stefin B 98 residues - 11124 Da.

1

MET

SER

GLY

ALA

PRO

SER

ALA

THR

GLN

2

PRO

ALA

THR

ALA

GLU

THR

GLN

HIS

ILE

ALA

3

ASP

GLN

VAL

ARG

SER

GLN

LEU

GLU

LYS

4

GLU

ASN

LYS

PHE

PRO

VAL

PHE

LYS

ALA

5

VAL

SER

PHE

LYS

SER

GLN

VAL

ALA

GLY

6

THR

ASN

TYR

PHE

ILE

LYS

VAL

HIS

VAL

GLY

7

ASP

GLU

ASP

PHE

VAL

HIS

LEU

ARG

VAL

PHE

8

GLN

SER

LEU

PRO

HIS

GLU

ASN

LYS

PRO

LEU

9

THR

LEU

SER

ASN

TYR

GLN

THR

ASN

LYS

ALA

10

LYS

HIS

ASP

GLU

LEU

THR

TYR

PHE

Samples:

sample_1: stefin B, [U-99% 13C; U-99% 15N], 1 mM; sodium phosphate 10 mM; sodium chloride 100 mM; H20 90%; D2O 10%

sample_conditions_1: ionic strength: 110 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1

Software:

FELIX v2004, Accelrys Software Inc. - data analysis

NMR spectrometers:

Bruker DRX 800 MHz
Bruker DRX 600 MHz
Bruker DRX 500 MHz

PDB	1STF 2OCT 4N6V
DBJ	BAA95541 BAC20304 BAC20305 BAC20306 BAC20307
EMBL	CAG46945
GB	AAA35727 AAA72786 AAA99014 AAF44059 AAH03370
REF	NP_000091 NP_001009095 XP_002830797 XP_011809620
SP	P04080 P60575 P60576 Q76LA0 Q8I030
AlphaFold	P04080 P60575 P60576 Q76LA0 Q8I030