BMRB Entry 11246

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11246
MolProbity Validation Chart

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Title:
Solution structure of the FHA domain of mouse Afadin 6
Deposition date:
2010-07-23
Original release date:
2011-08-03
Authors:
Hayashi, F.; Hirata, Y.; Guntert, P.; Yokoyama, S.
Citation:

Citation: Hayashi, F.; Hirata, Y.; Guntert, P.; Yokoyama, S.. "Solution structure of the FHA domain of mouse Afadin 6"  .

Assembly members:

Assembly members:
FHA domain, polymer, 120 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: house mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P020805-25

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FHA domain: GSSGSSGPEKLPYLVELSPD GSDSRDKPKLYRLQLSVTEV GTEKFDDNSIQLFGPGIQPH HCDLTNMDGVVTVTPRSMDA ETYVDGQRISETTMLQSGMR LQFGTSHVFKFVDPSGPSSG

Data sets:
Data typeCount
13C chemical shifts518
15N chemical shifts122
1H chemical shifts805

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1FHA domain1

Entities:

Entity 1, FHA domain 120 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYPROGLULYS
2   LEUPROTYRLEUVALGLULEUSERPROASP
3   GLYSERASPSERARGASPLYSPROLYSLEU
4   TYRARGLEUGLNLEUSERVALTHRGLUVAL
5   GLYTHRGLULYSPHEASPASPASNSERILE
6   GLNLEUPHEGLYPROGLYILEGLNPROHIS
7   HISCYSASPLEUTHRASNMETASPGLYVAL
8   VALTHRVALTHRPROARGSERMETASPALA
9   GLUTHRTYRVALASPGLYGLNARGILESER
10   GLUTHRTHRMETLEUGLNSERGLYMETARG
11   LEUGLNPHEGLYTHRSERHISVALPHELYS
12   PHEVALASPPROSERGLYPROSERSERGLY

Samples:

sample_1: FHA domain, [U-13C; U-15N], 0.7 mM; phosphate 20 mM; NaCl 100 mM; DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.902, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

PDB
GB EGW13515 EHA98723 ERE82130
REF XP_006523823 XP_006523825 XP_006523830 XP_006975980 XP_007607738

Download HSQC peak lists in one of the following formats:
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